Comparative analysis of the roles of HtrA-like surface proteases in two virulent Staphylococcus aureus strains.

Détails

ID Serval
serval:BIB_5157EE4B83BD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Comparative analysis of the roles of HtrA-like surface proteases in two virulent Staphylococcus aureus strains.
Périodique
Infection and Immunity
Auteur(s)
Rigoulay C., Entenza J.M., Halpern D., Widmer E., Moreillon P., Poquet I., Gruss A.
ISSN
0019-9567[print], 0019-9567[linking]
Statut éditorial
Publié
Date de publication
2005
Volume
73
Numéro
1
Pages
563-572
Langue
anglais
Résumé
The HtrA surface protease is involved in the virulence of many pathogens, mainly by its role in stress resistance and bacterial survival. Staphylococcus aureus encodes two putative HtrA-like proteases, referred to as HtrA(1) and HtrA(2). To investigate the roles of HtrA proteins in S. aureus, we constructed htrA(1), htrA(2), and htrA(1) htrA(2) insertion mutants in two genetically different virulent strains, RN6390 and COL. In the RN6390 context, htrA(1) inactivation resulted in sensitivity to puromycin-induced stress. The RN6390 htrA(1) htrA(2) mutant was affected in the expression of several secreted virulence factors comprising the agr regulon. This observation was correlated with the disappearance of the agr RNA III transcript in the RN6390 htrA(1) htrA(2) mutant. The virulence of this mutant was diminished in a rat model of endocarditis. In the COL context, both HtrA(1) and HtrA(2) were essential for thermal stress survival. However, only HtrA(1) had a slight effect on exoprotein expression. The htrA mutations did not diminish the virulence of the COL strain in the rat model of endocarditis. Our results indicate that HtrA proteins have different roles in S. aureus according to the strain, probably depending on specific differences in the regulation of virulence factor and stress protein expression. We propose that HtrA(1) and HtrA(2) contribute to pathogenicity by controlling the production of certain extracellular factors that are crucial for bacterial dissemination, as revealed in the RN6390 background. We speculate that HtrA proteins act in the agr-dependent regulation pathway by assuring folding and/or maturation of some surface components of the agr system.
Mots-clé
Animals, Bacterial Proteins/metabolism, Endocarditis, Bacterial/etiology, Endonucleases/metabolism, Genes, Regulator/physiology, Hemolysis, Micrococcal Nuclease/metabolism, Mitochondrial Proteins, Rats, Serine Endopeptidases/physiology, Staphylococcus aureus/enzymology, Staphylococcus aureus/genetics, Virulence Factors/analysis
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 14:45
Dernière modification de la notice
08/05/2019 18:34
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