Neonatal injection of native proton pump antigens induces autoimmune gastritis in mice

Détails

ID Serval
serval:BIB_515587ACF20D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Neonatal injection of native proton pump antigens induces autoimmune gastritis in mice
Périodique
Gastroenterology
Auteur(s)
Claeys  D., Saraga  E., Rossier  B. C., Kraehenbuhl  J. P.
ISSN
0016-5085
Statut éditorial
Publié
Date de publication
10/1997
Peer-reviewed
Oui
Volume
113
Numéro
4
Pages
1136-45
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
BACKGROUND & AIMS: Autoimmune gastritis is associated with gastric H+, K(+)-adenosine triphosphatase (ATPase)-specific autoantibodies (HKAb). The (auto) antigen that triggers disease and the pathogenic role of the autoantibodies are unknown. The aim of this study was to analyze when these autoantibodies are produced during autoimmune gastritis in neonatally thymectomized mice and whether a native H+, K(+)-ATPase antigen preparation can induce disease in mice. METHODS: Autoantibodies were characterized by a novel assay based on immunoprecipitation of a functional H+, K(+)-ATPase expressed in Xenopus oocytes. Normal mice were injected intraperitoneally with H+, K(+)-ATPase-enriched gastric membranes in the absence of adjuvant. RESULTS: Conformational autoantibodies recognizing both H+, K(+)-ATPase subunits appeared simultaneously with the gastric lesions 1 month after thymectomy. Immunization of neonates, but not adults, induced a persistent autoimmune gastritis in the body mucosa, characterized by lymphocytic infiltrations, loss of parietal and chief cells, metaplasia, and H+, K(+)-ATPase-specific autoantibodies. The histopathological lesions of this new model are similar to those in humans and thymectomized mice. CONCLUSIONS: The onset of gastritis and autoantibody production parallels the expression of the H+, K(+)-ATPase during ontogeny. Exposure of the neonatal immune system to organ-specific antigens expressed late after birth induces autoimmune gastritis in adult mice.
Mots-clé
Animals Animals, Newborn Antibody Formation Autoantibodies/*analysis Autoimmune Diseases/enzymology/*immunology/pathology CD4-Positive T-Lymphocytes/immunology/pathology CD8-Positive T-Lymphocytes/immunology/pathology Crosses, Genetic Female Gastric Mucosa/*enzymology/immunology/pathology Gastritis/enzymology/*immunology/pathology Immunoglobulin G/analysis Macromolecular Substances Male Mice Mice, Inbred Strains Oocytes/physiology Parietal Cells, Gastric/enzymology/immunology Proton Pumps/*immunology Swine Thymectomy Xenopus laevis
Pubmed
Web of science
Création de la notice
24/01/2008 14:01
Dernière modification de la notice
03/03/2018 17:10
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