Generation and characterization of malaria-specific human CD8(+) lymphocyte clones: effect of natural polymorphism on T cell recognition and endogenous cognate antigen presentationby liver cells

Details

Serval ID
serval:BIB_514D2CAAC142
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Generation and characterization of malaria-specific human CD8(+) lymphocyte clones: effect of natural polymorphism on T cell recognition and endogenous cognate antigen presentationby liver cells
Journal
European Journal of Immunology
Author(s)
Bonelo  A., Valmori  D., Triponez  F., Tiercy  J. M., Mentha  G., Oberholzer  J., Champagne  P., Romero  J. F., Esposito  F., Nebie  I., Barbey  C., Romero  P., Herrera  S., Corradin  G., Lopez  J. A.
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
11/2000
Volume
30
Number
11
Pages
3079-88
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
CD8(+) cytolytic T lymphocytes (CTL) play a fundamental role in the clearance of malaria parasites from the liver in mouse models. In humans, however, only low levels of parasite-specific CD8(+) T lymphocytes have been observed in individuals living in endemic areas. In the present study, we identified high levels of circulating CD8(+) T lymphocytes specific for a previously described HLA-A2-restricted CTL epitope of the circumsporozoite (CS) protein of Plasmodium falciparum in an adult living in Burkina Faso, as evidenced by IFN-gamma ELISPOT assay and MHC-tetramer technology. After cloning by limiting dilution culture, T cell recognition of natural CS variants of P. falciparum was studied. The results demonstrate that naturally occurring variations drastically affect residues critical for T cell recognition as only two out of nine sequences analyzed were efficiently recognized by the CTL clones. These clones were also used to analyze T cell recognition of the endogenously presented cognate antigen. We observed efficient antigen recognition of both HLA-A*0201-transfected murine antigen presenting cells and liver cells from HLA-A*0201/K(b)-transgenic mice upon infection with recombinant vaccinia virus encoding the CS protein (WR-CS). More importantly, we demonstrate for the first time efficient recognition of WR-CS-infected human liver cells.
Keywords
Adult Animals *Antigen Presentation/genetics Antigens, Protozoan/genetics/immunology CD8-Positive T-Lymphocytes/*immunology Cell Differentiation/immunology Cytotoxicity, Immunologic Humans Liver/*immunology Malaria/*immunology Mice Plasmodium falciparum/*immunology Polymorphism, Genetic/genetics Receptors, Antigen, T-Cell/genetics/immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:27
Last modification date
20/08/2019 14:07
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