Generation and characterization of malaria-specific human CD8(+) lymphocyte clones: effect of natural polymorphism on T cell recognition and endogenous cognate antigen presentationby liver cells
Details
Serval ID
serval:BIB_514D2CAAC142
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Generation and characterization of malaria-specific human CD8(+) lymphocyte clones: effect of natural polymorphism on T cell recognition and endogenous cognate antigen presentationby liver cells
Journal
European Journal of Immunology
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
11/2000
Volume
30
Number
11
Pages
3079-88
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
CD8(+) cytolytic T lymphocytes (CTL) play a fundamental role in the clearance of malaria parasites from the liver in mouse models. In humans, however, only low levels of parasite-specific CD8(+) T lymphocytes have been observed in individuals living in endemic areas. In the present study, we identified high levels of circulating CD8(+) T lymphocytes specific for a previously described HLA-A2-restricted CTL epitope of the circumsporozoite (CS) protein of Plasmodium falciparum in an adult living in Burkina Faso, as evidenced by IFN-gamma ELISPOT assay and MHC-tetramer technology. After cloning by limiting dilution culture, T cell recognition of natural CS variants of P. falciparum was studied. The results demonstrate that naturally occurring variations drastically affect residues critical for T cell recognition as only two out of nine sequences analyzed were efficiently recognized by the CTL clones. These clones were also used to analyze T cell recognition of the endogenously presented cognate antigen. We observed efficient antigen recognition of both HLA-A*0201-transfected murine antigen presenting cells and liver cells from HLA-A*0201/K(b)-transgenic mice upon infection with recombinant vaccinia virus encoding the CS protein (WR-CS). More importantly, we demonstrate for the first time efficient recognition of WR-CS-infected human liver cells.
Keywords
Adult
Animals
*Antigen Presentation/genetics
Antigens, Protozoan/genetics/immunology
CD8-Positive T-Lymphocytes/*immunology
Cell Differentiation/immunology
Cytotoxicity, Immunologic
Humans
Liver/*immunology
Malaria/*immunology
Mice
Plasmodium falciparum/*immunology
Polymorphism, Genetic/genetics
Receptors, Antigen, T-Cell/genetics/immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:27
Last modification date
20/08/2019 14:07