Systematic toxicological analysis using HPLC/DAD.

Détails

ID Serval
serval:BIB_512DE66EE9DC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Systematic toxicological analysis using HPLC/DAD.
Périodique
Journal of Forensic Sciences
Auteur(s)
Tracqui A., Kintz P., Mangin P.
ISSN
0022-1198[print], 0022-1198[linking]
Statut éditorial
Publié
Date de publication
1995
Volume
40
Numéro
2
Pages
254-262
Langue
anglais
Résumé
A high-performance liquid chromatographic method with diode-array detection (HPLC/DAD) for systematic toxicological analysis of human blood or plasma samples is presented. After single-step liquid/liquid extraction at pH 9.5 using chloroform/2-propanol/n-heptane (60:14:26, v/v/v), the drugs elute isocratically from a NovaPak C18 (Waters) 4-micrometers column (300 mm x 3.9 mm, i.d.) at 30 degrees C, with methanol/tetrahydrofuran/pH 2.6 phosphate buffer (65:5:30, v/v/v) as the mobile phase (flow rate 0.8 mL/min). Full UV spectra from 200 to 400 nm (resolution 1.3 nm) are recorded on-line during the 20 min chromatographic run. Solute identification may be automatically performed by comparison of analytical data (retention times and UV spectra) with references of 311 pharmaceuticals, toxicants and drugs of abuse stored in a computerized library. The method is simple, rapid, relatively inexpensive and highly specific. The previously reported applications of HPLC/DAD technology to drug screening are reviewed, and the interests and limitations of the method are discussed in the light of this literature.
Mots-clé
Chromatography, High Pressure Liquid/methods, Humans, Poisoning/blood, Poisoning/diagnosis, Poisons/blood, Toxicology/methods
Pubmed
Création de la notice
19/10/2010 16:27
Dernière modification de la notice
03/03/2018 17:10
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