G-418, an elongation inhibitor of 80 S ribosomes

Détails

ID Serval
serval:BIB_50FDFFD1C009
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
G-418, an elongation inhibitor of 80 S ribosomes
Périodique
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
Auteur(s)
Bar-Nun Shoshana, Shneyour Yonah, Beckmann Jacques S.
ISSN
0167-4781
ISSN-L
1879-2634
Statut éditorial
Publié
Date de publication
10/1983
Peer-reviewed
Oui
Volume
741
Numéro
1
Pages
123-127
Langue
anglais
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 13
Résumé
The mode of action of the aminoglycoside G-418 was studied in wheat-germ, cell-free translation systems programmed with rat-liver polyadenylated RNA. Incorporation of amino acids into protein was effectively inhibited by G-418 in the microM concentration range. The inhibition pattern obtained was not uniform. The synthesis of polypeptides with higher molecular weights was more inhibited than that of smaller polypeptides. An identical inhibition pattern within a similar range of concentrations was obtained with cycloheximide, a known elongation inhibitor. Translation activity was abolished when the wheat-germ 80 S ribosomes were removed and could be partially reconstructed upon addition of the ribosomes. Incubation with G-418 prior to isolation yielded ribosomes defective in their reconstruction ability. The inhibition pattern was not uniform and exhibited again the same relationship between the size of a polypeptide and the extent of inhibition of its synthesis. Therefore, we suggest that in wheat-germ, cell-free translation systems G-418 affects the 80 S ribosomes and inhibits the elongation cycle.
Mots-clé
Animals Anti-Bacterial Agents/*pharmacology Gentamicins/pharmacology Kinetics Liver/metabolism Peptide Chain Elongation, Translational/*drug effects Plants/metabolism Poly A/*genetics Protein Biosynthesis/drug effects RNA/*genetics RNA, Messenger Rats Ribosomes/*metabolism Triticum/metabolism, Biophysics, Genetics, Biochemistry, Structural Biology
Pubmed
Web of science
Création de la notice
25/01/2008 17:17
Dernière modification de la notice
03/03/2018 17:10
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