Munc18a controls SNARE assembly through its interaction with the syntaxin N-peptide.

Détails

ID Serval
serval:BIB_50EA0EA749D3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Munc18a controls SNARE assembly through its interaction with the syntaxin N-peptide.
Périodique
EMBO Journal
Auteur(s)
Burkhardt P., Hattendorf D.A., Weis W.I., Fasshauer D.
ISSN
1460-2075 (Electronic)
ISSN-L
0261-4189
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
27
Numéro
7
Pages
923-933
Langue
anglais
Résumé
Sec1/Munc18-like (SM) proteins functionally interact with SNARE proteins in vesicular fusion. Despite their high sequence conservation, structurally disparate binding modes for SM proteins with syntaxins have been observed. Several SM proteins appear to bind only to a short peptide present at the N terminus of syntaxin, designated the N-peptide, while Munc18a binds to a 'closed' conformation formed by the remaining portion of syntaxin 1a. Here, we show that the syntaxin 16 N-peptide binds to the SM protein Vps45, but the remainder of syntaxin 16 strongly enhances the affinity of the interaction. Likewise, the N-peptide of syntaxin 1a serves as a second binding site in the Munc18a/syntaxin 1a complex. When the syntaxin 1a N-peptide is bound to Munc18a, SNARE complex formation is blocked. Removal of the N-peptide enables binding of syntaxin 1a to its partner SNARE SNAP-25, while still bound to Munc18a. This suggests that Munc18a controls the accessibility of syntaxin 1a to its partners, a role that might be common to all SM proteins.
Mots-clé
Animals, Binding Sites, Calorimetry, Crystallography, X-Ray, Membrane Proteins/metabolism, Models, Molecular, Munc18 Proteins/metabolism, Mutant Proteins/metabolism, Peptides/metabolism, Protein Binding, Protein Structure, Secondary, Rats, SNARE Proteins/metabolism, Syntaxin 1/chemistry, Syntaxin 1/metabolism, Thermodynamics, Vesicular Transport Proteins/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/09/2011 9:07
Dernière modification de la notice
08/05/2019 18:32
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