Variations of plasmid content in Rickettsia felis.

Détails

Ressource 1Télécharger: BIB_50860BC22A74.P001.pdf (196.86 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_50860BC22A74
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Variations of plasmid content in Rickettsia felis.
Périodique
Plos One
Auteur(s)
Fournier P.E., Belghazi L., Robert C., Elkarkouri K., Richards A.L., Greub G., Collyn F., Ogawa M., Portillo A., Oteo J.A., Psaroulaki A., Bitam I., Raoult D.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
3
Numéro
5
Pages
e2289
Langue
anglais
Résumé
BACKGROUND: Since its first detection, characterization of R. felis has been a matter of debate, mostly due to the contamination of an initial R. felis culture by R. typhi. However, the first stable culture of R. felis allowed its precise phenotypic and genotypic characterization, and demonstrated that this species belonged to the spotted fever group rickettsiae. Later, its genome sequence revealed the presence of two forms of the same plasmid, physically confirmed by biological data. In a recent article, Gillespie et al. (PLoS One. 2007;2(3):e266.) used a bioinformatic approach to refute the presence of the second plasmid form, and proposed the creation of a specific phylogenetic group for R. felis.
METHODOLOGY/PRINCIPAL FINDINGS: In the present report, we, and five independent international laboratories confirmed unambiguously by PCR the presence of two plasmid forms in R. felis strain URRWXCal(2) (T), but observed that the plasmid content of this species, from none to 2 plasmid forms, may depend on the culture passage history of the studied strain. We also demonstrated that R. felis does not cultivate in Vero cells at 37 degrees C but generates plaques at 30 degrees C. Finally, using a phylogenetic study based on 667 concatenated core genes, we demonstrated the position of R. felis within the spotted fever group.
SIGNIFICANCE: We demonstrated that R. felis, which unambiguously belongs to the spotted fever group rickettsiae, may contain up to two plasmid forms but this plasmid content is unstable.
Mots-clé
Base Sequence, DNA Primers, Phylogeny, Plasmids, Polymerase Chain Reaction, Rickettsia felis/classification, Rickettsia felis/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
03/04/2016 22:30
Dernière modification de la notice
08/05/2019 18:31
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