Origin and Evolution of the Bartonella Gene Transfer Agent.

Détails

ID Serval
serval:BIB_506D6C3BBA94
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Origin and Evolution of the Bartonella Gene Transfer Agent.
Périodique
Molecular biology and evolution
Auteur(s)
Tamarit D., Neuvonen M.M., Engel P., Guy L., Andersson SGE
ISSN
1537-1719 (Electronic)
ISSN-L
0737-4038
Statut éditorial
Publié
Date de publication
01/02/2018
Peer-reviewed
Oui
Volume
35
Numéro
2
Pages
451-464
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Gene transfer agents (GTAs) are domesticated bacteriophages that have evolved into molecular machines for the transfer of bacterial DNA. Despite their widespread nature and their biological implications, the mechanisms and selective forces that drive the emergence of GTAs are still poorly understood. Two GTAs have been identified in the Alphaproteobacteria: the RcGTA, which is widely distributed in a broad range of species; and the BaGTA, which has a restricted host range that includes vector-borne intracellular bacteria of the genus Bartonella. The RcGTA packages chromosomal DNA randomly, whereas the BaGTA particles contain a relatively higher fraction of genes for host interaction factors that are amplified from a nearby phage-derived origin of replication. In this study, we compare the BaGTA genes with homologous bacteriophage genes identified in the genomes of Bartonella species and close relatives. Unlike the BaGTA, the prophage genes are neither present in all species, nor inserted into homologous genomic sites. Phylogenetic inferences and substitution frequency analyses confirm codivergence of the BaGTA with the host genome, as opposed to multiple integration and recombination events in the prophages. Furthermore, the organization of segments flanking the BaGTA differs from that of the prophages by a few rearrangement events, which have abolished the normal coordination between phage genome replication and phage gene expression. Based on the results of our comparative analysis, we propose a model for how a prophage may be transformed into a GTA that transfers amplified bacterial DNA segments.
Mots-clé
Bartonella/genetics, Bartonella/virology, Biological Evolution, Gene Amplification, Gene Transfer, Horizontal, Genome, Bacterial, Inheritance Patterns, Lysogeny, Models, Genetic, Prophages/physiology, Virus Replication, Bartonella, bacteriophage, gene transfer agent, horizontal gene transfer
Pubmed
Web of science
Création de la notice
23/11/2017 19:27
Dernière modification de la notice
01/04/2019 6:26
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