Cytochrome c oxidase expression in chronic and intermittent hypoxia rat gastrocnemius muscle quantitated by CE

Détails

ID Serval
serval:BIB_5031531CA746
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cytochrome c oxidase expression in chronic and intermittent hypoxia rat gastrocnemius muscle quantitated by CE
Périodique
Electrophoresis
Auteur(s)
Ripamonti  M., Vigano  A., Moriggi  M., Milano  G., von Segesser  L. K., Samaja  M., Gelfi  C.
ISSN
0173-0835
Statut éditorial
Publié
Date de publication
10/2006
Peer-reviewed
Oui
Volume
27
Numéro
19
Pages
3897-903
Notes
Journal Article --- Old month value: Oct
Résumé
We investigated the effects of hypoxia and intermittent hypoxia in rat muscle by quantitating the expression of genes encoding cytochrome c oxidase (CytOx) subunits I, II and IV, and ribosomal 12S RNA. The quantitative assessment was made by CE in a polymer network, laser being used to facilitate the detection of induced fluorescence. Constant and intermittent hypoxia influence gene expression and ribosomal activity to different degrees. We found constant hypoxia to be accompanied by an increment in the mitochondrial CytOx subunit transcripts II and I and 12S ribosomal subunit, whereas the nuclear subunit (IV) remained unchanged. No changes were observed in intermittent hypoxic rats. Despite the increment in messenger expression, the decrease in enzyme activity was accompanied by a decrease in citrate synthase activity, a marker of mitochondrial function. The increment in CytOx mitochondrial subunits messengers and ribosomal 12S RNA under prolonged chronic hypoxia could be a consequence of reduced protein synthesis that leads to messenger accumulation.
Mots-clé
Animals Anoxia/*enzymology/genetics Electron Transport Complex IV/*genetics Electrophoresis, Capillary/*methods *Gene Expression Regulation, Enzymologic Lasers Male Mitochondria/enzymology Muscle, Skeletal/*enzymology RNA, Messenger/analysis/metabolism Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction Spectrometry, Fluorescence
Pubmed
Web of science
Création de la notice
18/01/2008 15:38
Dernière modification de la notice
03/03/2018 17:08
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