Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta.

Détails

ID Serval
serval:BIB_4F0D4E9107E3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta.
Périodique
Insect Biochemistry and Molecular Biology
Auteur(s)
Kanost M.R., Arrese E.L., Cao X., Chen Y.R., Chellapilla S., Goldsmith M.R., Grosse-Wilde E., Heckel D.G., Herndon N., Jiang H., Papanicolaou A., Qu J., Soulages J.L., Vogel H., Walters J., Waterhouse R.M., Ahn S.J., Almeida F.C., An C., Aqrawi P., Bretschneider A., Bryant W.B., Bucks S., Chao H., Chevignon G., Christen J.M., Clarke D.F., Dittmer N.T., Ferguson LCF, Garavelou S., Gordon KHJ, Gunaratna R.T., Han Y., Hauser F., He Y., Heidel-Fischer H., Hirsh A., Hu Y., Jiang H., Kalra D., Klinner C., König C., Kovar C., Kroll A.R., Kuwar S.S., Lee S.L., Lehman R., Li K., Li Z., Liang H., Lovelace S., Lu Z., Mansfield J.H., McCulloch K.J., Mathew T., Morton B., Muzny D.M., Neunemann D., Ongeri F., Pauchet Y., Pu L.L., Pyrousis I., Rao X.J., Redding A., Roesel C., Sanchez-Gracia A., Schaack S., Shukla A., Tetreau G., Wang Y., Xiong G.H., Traut W., Walsh T.K., Worley K.C., Wu D., Wu W., Wu Y.Q., Zhang X., Zou Z., Zucker H., Briscoe A.D., Burmester T., Clem R.J., Feyereisen R., Grimmelikhuijzen CJP, Hamodrakas S.J., Hansson B.S., Huguet E., Jermiin L.S., Lan Q., Lehman H.K., Lorenzen M., Merzendorfer H., Michalopoulos I., Morton D.B., Muthukrishnan S., Oakeshott J.G., Palmer W., Park Y., Passarelli A.L., Rozas J., Schwartz L.M., Smith W., Southgate A., Vilcinskas A., Vogt R., Wang P., Werren J., Yu X.Q., Zhou J.J., Brown S.J., Scherer S.E., Richards S., Blissard G.W.
ISSN
1879-0240 (Electronic)
ISSN-L
0965-1748
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
76
Pages
118-147
Langue
anglais
Résumé
Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects.

Mots-clé
Animals, Gene Expression, Gene Expression Profiling, Genome, Insect, Larva/genetics, Larva/growth & development, Manduca/genetics, Manduca/growth & development, Pupa/genetics, Pupa/growth & development, Sequence Analysis, DNA, Synteny, Innate immunity, Insect, Insect biochemistry, Lepidoptera, Moth, Tobacco hornworm
Pubmed
Web of science
Création de la notice
20/09/2017 9:53
Dernière modification de la notice
03/03/2018 17:06
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