SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III.
Details
Serval ID
serval:BIB_4ED2D02DF1AA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III.
Journal
Genes and Development
ISSN
0890-9369[print], 0890-9369[linking]
Publication state
Published
Issued date
09/1998
Volume
12
Number
17
Pages
2664-2672
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Publication Status: ppublish
Abstract
The basal transcription factor SNAPc binds to the PSE, a core element in the RNA polymerase II and III human snRNA promoters. SNAPc contains at least four subunits, but it has not been possible to assemble a fully defined recombinant SNAPc. Here we reconstitute SNAPc from five recombinant subunits, SNAP43, SNAP45, SNAP50, SNAP190, and a newly identified subunit, SNAP19. This recombinant complex binds specifically to the PSE and directs both RNA polymerase II and III snRNA gene transcription. Thus, the same core SNAPc nucleates the assembly of two classes of initiation complexes.
Keywords
Amino Acid Sequence, Base Sequence, Hela Cells, Humans, Macromolecular Substances, Models, Molecular, Molecular Sequence Data, Promoter Regions, Genetic, RNA Polymerase II/metabolism, RNA Polymerase III/metabolism, RNA, Small Nuclear/genetics, RNA, Small Nuclear/metabolism, Recombinant Proteins/biosynthesis, Recombinant Proteins/chemistry, Transcription Factors/biosynthesis, Transcription Factors/chemistry, Transcription, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
21/01/2008 16:34
Last modification date
20/08/2019 14:04
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