Clusterin in renal tissue: preferential localization with the terminal complement complex and immunoglobulin deposits in glomeruli

Details

Serval ID
serval:BIB_4E7CC2FB3DB4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Clusterin in renal tissue: preferential localization with the terminal complement complex and immunoglobulin deposits in glomeruli
Journal
Clinical and Experimental Immunology
Author(s)
French  L. E., Tschopp  J., Schifferli  J. A.
ISSN
0009-9104 (Print)
Publication state
Published
Issued date
06/1992
Volume
88
Number
3
Pages
389-93
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
The membrane attack complex (MAC) of complement is activated by immune and non-immune mechanisms in the kidney. MAC has been found associated with glomerular immune deposits, but also to cell remnants, particularly along tubules and in vessel walls. Clusterin and S-protein (vitronectin) bind to MAC, rendering it cytolytically inactive. Both have been found associated with MAC in renal tissue. Here we analysed the deposition of clusterin and S-protein in 118 renal biopsies relative to the localization of the MAC using MoAbs. Statistical analysis was performed comparing no or little versus evident or strong staining by immunofluorescence (IF). In glomeruli, out of the 92 biopsies where both MAC and immunoglobulins were evaluated, deposits of MAC were found in the presence (32 out of 41) but also in the absence of immunoglobulins (20/51). Clusterin and S-protein deposits were seen, respectively, in 25 out of 61 and 36 out of 61 biopsies containing glomerular MAC, and almost never in its absence (one out of 50 for both). The association of the two inhibitors with MAC was observed mainly in glomeruli containing immunoglobulin deposits (respectively, 21 out of 32 and 25 out of 32), but not when immunoglobulins were absent (three out of 20 and seven out of 20) (coefficient of concordance, K = 0.47 and 0.43). The localization of MAC along tubules and in vessels was easily identified in most biopsies (93 out of 118) and was accompanied by S-protein in most cases (tubules, 86 out of 93; vessels, 82 out of 93) (K = 0.58 and 0.57 respectively) but not by clusterin (28 out of 93 and 24 out of 93). These results suggest that clusterin does not co-localize with MAC whenever there is formation and fixation of the MAC. It seems that clusterin has a particular affinity for MAC which is associated with immunoglobulin. This observation should help to distinguish between the different forms of MAC, and might indicate that MAC associated with immunoglobulin is essentially in its cytolytically inactive form.
Keywords
Biopsy Clusterin Complement C3/biosynthesis Complement Membrane Attack Complex/biosynthesis Fluorescent Antibody Technique Glycoproteins/*biosynthesis Humans Kidney/*metabolism Membrane Glycoproteins/biosynthesis *Molecular Chaperones
Pubmed
Web of science
Create date
24/01/2008 15:18
Last modification date
20/08/2019 14:04
Usage data