Luminal particles within cellular microtubules.

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Serval ID
serval:BIB_4DCBF54C0BDB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Luminal particles within cellular microtubules.
Journal
Journal of Cell Biology
Author(s)
Garvalov B.K., Zuber B., Bouchet-Marquis C., Kudryashev M., Gruska M., Beck M., Leis A., Frischknecht F., Bradke F., Baumeister W., Dubochet J., Cyrklaff M.
ISSN
0021-9525
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
174
Number
6
Pages
759-765
Language
english
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Sep 11
Abstract
The regulation of microtubule dynamics is attributed to microtubule-associated proteins that bind to the microtubule outer surface, but little is known about cellular components that may associate with the internal side of microtubules. We used cryoelectron tomography to investigate in a quantitative manner the three dimensional structure of microtubules in intact mammalian cells. We show that the lumen of microtubules in this native state is filled with discrete, globular particles with a diameter of 7 nm and spacings between 8 and 20 nm in neuronal cells. Cross-sectional views of microtubules confirm the presence of luminal material in vitreous sections of brain tissue. Most of the luminal particles had connections to the microtubule wall, as revealed in tomograms. A higher accumulation of particles was seen near the retracting plus ends of microtubules. The luminal particles were abundant in neurons, but were also observed in other cells, such as astrocytes and stem cells.
Keywords
Animals, Animals, Newborn, Astrocytes, Axons, Cells, Cultured, Cryoelectron Microscopy, Cytoplasmic Granules, Hela Cells, Hippocampus, Humans, Mice, Microtubule-Associated Proteins, Microtubules, Neurites, Neurons, Organ Culture Techniques, Pluripotent Stem Cells, Rats
Pubmed
Web of science
Create date
24/01/2008 10:25
Last modification date
20/08/2019 14:02
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