The structure of cell wall alpha-glucan from fission yeast.

Détails

ID Serval
serval:BIB_4D06B909018E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The structure of cell wall alpha-glucan from fission yeast.
Périodique
Glycobiology
Auteur(s)
Grün C.H., Hochstenbach F., Humbel B.M., Verkleij A.J., Sietsma J.H., Klis F.M., Kamerling J.P., Vliegenthart J.F.
ISSN
0959-6658 (Print)
ISSN-L
0959-6658
Statut éditorial
Publié
Date de publication
2005
Volume
15
Numéro
3
Pages
245-257
Langue
anglais
Résumé
Morphology and structural integrity of fungal cells depend on cell wall polysaccharides. The chemical structure and biosynthesis of two types of these polysaccharides, chitin and (1-->3)-beta-glucan, have been studied extensively, whereas little is known about alpha-glucan. Here we describe the chemical structure of alpha-glucan isolated from wild-type and mutant cell walls of the fission yeast Schizosaccharomyces pombe. Wild-type alpha-glucan was found to consist of a single population of linear glucose polymers, approximately 260 residues in length. These glucose polymers were composed of two interconnected linear chains, each consisting of approximately 120 (1-->3)-linked alpha-d-glucose residues and some (1-->4)-linked alpha-D-glucose residues at the reducing end. By contrast, alpha-glucan of an alpha-glucan synthase mutant with an aberrant cell morphology and reduced alpha-glucan levels consisted of a single chain only. We propose that alpha-glucan biosynthesis involves an ordered series of events, whereby two alpha-glucan chains are coupled to create mature cell wall alpha-glucan. This mature form of cell wall alpha-glucan is essential for fission-yeast morphogenesis.
Mots-clé
Carbohydrate Sequence, Cell Wall/chemistry, Cell Wall/genetics, Glucans/chemistry, Glucans/genetics, Glucose/metabolism, Magnetic Resonance Spectroscopy, Microscopy, Electron, Transmission, Molecular Sequence Data, Mutation/genetics, Schizosaccharomyces/chemistry, Schizosaccharomyces/cytology
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/10/2012 15:19
Dernière modification de la notice
08/05/2019 18:19
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