Division of Listeria monocytogenes serovar 4b strains into two groups by PCR and restriction enzyme analysis

Details

Serval ID
serval:BIB_4C93599A9472
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Division of Listeria monocytogenes serovar 4b strains into two groups by PCR and restriction enzyme analysis
Journal
Applied and Environmental Microbiology
Author(s)
Ericsson  H., Stalhandske  P., Danielsson-Tham  M. L., Bannerman  E., Bille  J., Jacquet  C., Rocourt  J., Tham  W.
ISSN
0099-2240
Publication state
Published
Issued date
11/1995
Peer-reviewed
Oui
Volume
61
Number
11
Pages
3872-4
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
Altogether, 133 strains of Listeria monocytogenes serovar 4b were investigated. A segment of 2,916 bp containing parts of the two genes inlA and inlB in L. monocytogenes was amplified by the PCR technique. The PCR product obtained was cleaved with the restriction enzyme AluI, and the fragments generated were separated by gel electrophoresis, leading to two distinct groups: PCR-restriction enzyme analysis groups I and II, containing 37 and 96 strains, respectively. The PCR-restriction enzyme analysis method described in this paper could be a useful tool for the subtyping of L. monocytogenes serovar 4b strains.
Keywords
Animals Bacterial Typing Techniques Base Sequence DNA Primers/genetics DNA Restriction Enzymes DNA, Bacterial/genetics Humans Listeria monocytogenes/*classification/*genetics/pathogenicity Molecular Sequence Data Polymerase Chain Reaction Serotyping Virulence
Pubmed
Web of science
Create date
11/02/2008 12:39
Last modification date
20/08/2019 14:01
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