Simultaneous determination of glafenine and floctafenine in human plasma using high-performance liquid chromatography.
Details
Serval ID
serval:BIB_4C0CDAF56AE8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Simultaneous determination of glafenine and floctafenine in human plasma using high-performance liquid chromatography.
Journal
Annales De Biologie Clinique
ISSN
0003-3898[print], 0003-3898[linking]
Publication state
Published
Issued date
1988
Volume
46
Number
8
Pages
665-667
Language
english
Abstract
A high-performance liquid chromatography (HPLC) assay has been developed for the determination of glafenine and floctafenine in plasma. Drugs are extracted with ethyl acetate from alkalinized samples using antipyrine as an internal standard. After evaporating the solvent to dryness, the residue is removed and analyzed using a microPorasil 10 micron column with a mobile phase of n-hexane and ethanol (80/20, v/v). The drugs are detected at 240 nm. The lower detection limits are 1.993.10(-2) mumol/l and 3.138.10(-2) mumol/l for glafenine and floctafenine, respectively. The method is simple, rapid and very sensitive. It is the first HPLC method for the determination of floctafenine.
Keywords
Anthranilic Acids/blood, Antipyrine, Chromatography, High Pressure Liquid/methods, Ethanol, Glafenine/blood, Hexanes, Humans, Time Factors
Pubmed
Create date
14/10/2010 16:57
Last modification date
20/08/2019 15:00
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