Two waves of recombinase gene expression in developing thymocytes.

Détails

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Etat: Public
Version: Final published version
Licence: CC BY-NC-SA 4.0
ID Serval
serval:BIB_4B7BC91A7C1B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Two waves of recombinase gene expression in developing thymocytes.
Périodique
The Journal of experimental medicine
Auteur(s)
Wilson A., Held W., MacDonald H.R.
ISSN
0022-1007
Statut éditorial
Publié
Date de publication
1994
Peer-reviewed
Oui
Volume
179
Numéro
4
Pages
1355-1360
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
During T cell development in the thymus, T cell receptor (TCR) alpha, beta, gamma, and delta genes are rearranged and expressed. TCR rearrangement strictly depends upon the coordinate activity of two recombinase activating genes, Rag-1 and Rag-2. In this study we have followed the expression of these genes at different stages of intrathymic development. The results indicate that there are two periods of high Rag-1 and Rag-2 mRNA expression. The first wave peaks early at the CD25+CD4-CD8-CD3- stage of development and coincides with the initial appearance of transcripts derived from fully rearranged TCR beta, gamma, and delta genes, whereas the second wave occurs later at the CD4+CD8+ stage coincident with full-length TCR alpha mRNA expression. Active downregulation of Rag-1 and Rag-2 mRNA expression appears to occur in vivo between the two peaks of recombinase activity. This phenomenon can be mimicked in vitro in response to artificial stimuli such as phorbol myristate acetate and calcium ionophore. Collectively our data suggest that recombinase expression is actively regulated during early thymus development independently of cell surface expression of a mature heterodimeric TCR protein complex.
Mots-clé
Animals, DNA Nucleotidyltransferases/biosynthesis, DNA Nucleotidyltransferases/genetics, DNA-Binding Proteins, Female, Gene Expression Regulation, Enzymologic, Homeodomain Proteins, Integrases, Mice, Mice, Inbred C57BL, Protein Biosynthesis, Proteins/genetics, Receptors, Antigen, T-Cell/biosynthesis, Recombinases, T-Lymphocyte Subsets/enzymology, T-Lymphocyte Subsets/metabolism, Thymus Gland/cytology, Thymus Gland/enzymology
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/01/2008 15:24
Dernière modification de la notice
20/08/2019 13:59
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