High capacity in G protein-coupled receptor signaling.

Détails

Ressource 1Télécharger: s41467-018-02868-y.pdf (1167.42 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_4B5E271BB510
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
High capacity in G protein-coupled receptor signaling.
Périodique
Nature communications
Auteur(s)
Keshelava A., Solis G.P., Hersch M., Koval A., Kryuchkov M., Bergmann S., Katanaev V.L.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Statut éditorial
Publié
Date de publication
28/02/2018
Peer-reviewed
Oui
Volume
9
Numéro
1
Pages
876
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
G protein-coupled receptors (GPCRs) constitute a large family of receptors that activate intracellular signaling pathways upon detecting specific extracellular ligands. While many aspects of GPCR signaling have been uncovered through decades of studies, some fundamental properties, like its channel capacity-a measure of how much information a given transmission system can reliably transduce-are still debated. Previous studies concluded that GPCRs in individual cells could transmit around one bit of information about the concentration of the ligands, allowing only for a reliable on or off response. Using muscarinic receptor-induced calcium response measured in individual cells upon repeated stimulation, we show that GPCR signaling systems possess a significantly higher capacity. We estimate the channel capacity of this system to be above two, implying that at least four concentration levels of the agonist can be distinguished reliably. These findings shed light on the basic principles of GPCR signaling.
Mots-clé
Acetylcholine/pharmacology, Calcium/metabolism, Cell Line, Cell Membrane/metabolism, HEK293 Cells, Humans, Muscarinic Agonists/pharmacology, Receptor, Muscarinic M3/metabolism, Receptors, G-Protein-Coupled/agonists, Receptors, G-Protein-Coupled/metabolism, Signal Transduction/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/03/2018 10:48
Dernière modification de la notice
20/08/2019 13:59
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