Characterisation of dic(9;20)(p11-13;q11) in childhood B-cell precursor acute lymphoblastic leukaemia by tiling resolution array-based comparative genomic hybridisation reveals clustered breakpoints at 9p13.2 and 20q11.2.

Details

Serval ID
serval:BIB_4B4AA01EA0FC
Type
Article: article from journal or magazin.
Collection
Publications
Title
Characterisation of dic(9;20)(p11-13;q11) in childhood B-cell precursor acute lymphoblastic leukaemia by tiling resolution array-based comparative genomic hybridisation reveals clustered breakpoints at 9p13.2 and 20q11.2.
Journal
British Journal of Haematology
Author(s)
Schoumans J., Johansson B., Corcoran M., Kuchinskaya E., Golovleva I., Grandér D., Forestier E., Staaf J., Borg A., Gustafsson B., Blennow E., Nordgren A.
ISSN
0007-1048 (Print)
ISSN-L
0007-1048
Publication state
Published
Issued date
2006
Volume
135
Number
4
Pages
492-499
Language
english
Notes
Publication types: Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Although the dic(9;20)(p11-13;q11) is a recurrent chromosomal abnormality in paediatric B-cell precursor acute lymphoblastic leukaemia (BCP ALL), occurring in approximately 2% of the cases, its molecular genetic consequences have not been elucidated. In the present study, high-resolution genome-wide array-based comparative genomic hybridisation (array-CGH) and fluorescence in situ hybridisation (FISH) were used to characterise the 9p and 20q breakpoints (BPs) in seven childhood BCP ALLs with dic(9;20), which was shown to be unbalanced in all of them, resulting in loss of 9p13.2-pter. Five of the cases had loss of 20q11.2-qter, whereas two displayed gain of 20cen-pter. All BPs on 9p clustered in a 1.5 Mb segment of the sub-band 9p13.2; in three of the cases, the 20q BPs mapped to three adjacent clones covering a distance of 350 kb at 20q11.2. Thus, the aberration should be designated dic(9;20)(p13.2;q11.2). One of the ALLs, shown to have a complex dic(9;20), was further investigated by FISH, revealing a rearrangement of the haemapoietic cell kinase isoform p61 (HCK) gene at 20q11. The disruption of HCK may result in a fusion gene or in loss of function. Unfortunately, lack of material precluded further analyses of HCK. Thus, it remains to be elucidated whether dic(9;20)(p13.2;q11.2) leads to a chimaeric gene or whether the functionally important outcome is loss of 9p and 20q material.
Keywords
Adolescent, Burkitt Lymphoma/genetics, Child, Child, Preschool, Chromosome Aberrations, Chromosome Deletion, Chromosome Mapping/methods, Chromosomes, Human, Pair 20/genetics, Chromosomes, Human, Pair 9/genetics, DNA, Neoplasm/genetics, Female, Humans, In Situ Hybridization, Fluorescence/methods, Infant, Karyotyping, Male, Nucleic Acid Hybridization/methods, Oligonucleotide Array Sequence Analysis/methods
Pubmed
Web of science
Open Access
Yes
Create date
17/09/2011 9:28
Last modification date
20/08/2019 13:59
Usage data