Noninvasive measurement of cardiovascular function in mice with high-temporal-resolution small-animal PET

Details

Serval ID
serval:BIB_4B3668DCE5FC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Noninvasive measurement of cardiovascular function in mice with high-temporal-resolution small-animal PET
Journal
Journal of Nuclear Medicine
Author(s)
Kreissl  M. C., Wu  H. M., Stout  D. B., Ladno  W., Schindler  T. H., Zhang  X., Prior  J. O., Prins  M. L., Chatziioannou  A. F., Huang  S. C., Schelbert  H. R.
ISSN
0161-5505
Publication state
Published
Issued date
06/2006
Peer-reviewed
Oui
Volume
47
Number
6
Pages
974-80
Notes
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S. --- Old month value: Jun
Abstract
The aim of this study was to explore the feasibility of determining parameters of cardiovascular function in mice noninvasively by high-temporal-resolution imaging with a dedicated small-animal PET system. METHODS: Twenty-five anesthetized mice (28.8 +/- 4.6 g) were injected via an intravenous catheter with a 30-microL bolus of (18)F-FDG (8-44 MBq). The first 9 s of data were reconstructed into 30 frames of 0.3 s using filtered backprojection. The time-activity curve derived from a left ventricle volume of interest was corrected for tracer recirculation and partial volume. Cardiac output was calculated by the Stewart-Hamilton method, in which cardiac output is total injected activity divided by the area under the left ventricle time-activity curve. Cardiac output divided by body weight was defined as cardiac index; cardiac output divided by heart rate yielded the stroke volume. In 5 mice, measurements were repeated 2-4 times to assess reproducibility. In 4 mice, the hemodynamic response to dobutamine was examined by measuring heart rate, cardiac output, and stroke volume. RESULTS: The cardiac output averaged 20.4 +/- 3.4 mL/min; in the repeated measurements, the parameter displayed a mean percentage SD per mouse of 10% +/- 6%. The cardiac index averaged 0.73 +/- 0.19 mL/min/g and the stroke volume 45.0 +/- 6.9 microL, and both correlated with heart rate (r = 0.53, P = 0.007, and r = 0.49, P = 0.01, respectively). During dobutamine stress, heart rate increased from 423 +/- 50 to 603 +/- 30 beats/min (P = 0.002) and cardiac output increased from 18.5 +/- 1.9 to 32.0 +/- 4.2 mL/min (P = 0.008). CONCLUSION: Parameters of cardiovascular function can be measured in mice noninvasively by radionuclide angiography using high-temporal-resolution small-animal PET. Measured values of cardiac output and stroke volume are reproducible and comparable to those obtained with MRI. The approach permits the monitoring of changes in cardiovascular function in response to pharmacologic intervention.
Keywords
Animals Cardiac Volume/*physiology Feasibility Studies Heart Ventricles/*physiology/*radionuclide imaging Image Interpretation, Computer-Assisted/*methods Male Mice Mice, Inbred C57BL Positron-Emission Tomography/methods/*veterinary Reproducibility of Results Sensitivity and Specificity Stroke Volume/*physiology
Pubmed
Web of science
Create date
29/01/2008 17:38
Last modification date
20/08/2019 14:59
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