Regulation of yeast chronological life span by TORC1 via adaptive mitochondrial ROS signaling.

Details

Serval ID
serval:BIB_4AE667AD56FD
Type
Article: article from journal or magazin.
Collection
Publications
Title
Regulation of yeast chronological life span by TORC1 via adaptive mitochondrial ROS signaling.
Journal
Cell metabolism
Author(s)
Pan Y., Schroeder E.A., Ocampo A., Barrientos A., Shadel G.S.
ISSN
1932-7420 (Electronic)
ISSN-L
1550-4131
Publication state
Published
Issued date
08/06/2011
Peer-reviewed
Oui
Volume
13
Number
6
Pages
668-678
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Here we show that yeast strains with reduced target of rapamycin (TOR) signaling have greater overall mitochondrial electron transport chain activity during growth that is efficiently coupled to ATP production. This metabolic alteration increases mitochondrial membrane potential and reactive oxygen species (ROS) production, which we propose supplies an adaptive signal during growth that extends chronological life span (CLS). In strong support of this concept, uncoupling respiration during growth or increasing expression of mitochondrial manganese superoxide dismutase significantly curtails CLS extension in tor1Δ strains, and treatment of wild-type strains with either rapamycin (to inhibit TORC1) or menadione (to generate mitochondrial ROS) during growth is sufficient to extend CLS. Finally, extension of CLS by reduced TORC1/Sch9p-mitochondrial signaling occurs independently of Rim15p and is not a function of changes in media acidification/composition. Considering the conservation of TOR-pathway effects on life span, mitochondrial ROS signaling may be an important mechanism of longevity regulation in higher organisms.
Keywords
Adaptation, Physiological/drug effects, Colony Count, Microbial, Dinitrophenols/pharmacology, Gene Knockout Techniques, Membrane Potential, Mitochondrial/drug effects, Membrane Potential, Mitochondrial/genetics, Microbiological Phenomena/drug effects, Microbiological Phenomena/genetics, Mitochondria/drug effects, Mitochondria/metabolism, Multiprotein Complexes/genetics, Multiprotein Complexes/metabolism, Oxygen Consumption/genetics, Phosphatidylinositol 3-Kinases/antagonists & inhibitors, Phosphatidylinositol 3-Kinases/genetics, Phosphatidylinositol 3-Kinases/metabolism, Protein Kinases/genetics, Protein Kinases/metabolism, Protein-Serine-Threonine Kinases/genetics, Protein-Serine-Threonine Kinases/metabolism, Saccharomyces cerevisiae/drug effects, Saccharomyces cerevisiae/growth & development, Saccharomyces cerevisiae/physiology, Saccharomyces cerevisiae Proteins/antagonists & inhibitors, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism, Sirolimus/pharmacology, Superoxides/metabolism, Vitamin K 3/pharmacology
Pubmed
Web of science
Open Access
Yes
Create date
14/08/2018 10:48
Last modification date
20/08/2019 14:58
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