Functional expression of mammalian receptors and membrane channels in different cells.

Details

Serval ID
serval:BIB_4A4E11EC43C3
Type
Article: article from journal or magazin.
Collection
Publications
Title
Functional expression of mammalian receptors and membrane channels in different cells.
Journal
Journal of Structural Biology
Author(s)
Eifler N., Duckely M., Sumanovski L.T., Egan T.M., Oksche A., Konopka J.B., Lüthi A., Engel A., Werten P.J.
ISSN
1047-8477
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
159
Number
2
Pages
179-193
Language
english
Abstract
In native tissues, the majority of medically important membrane proteins is only present at low concentrations, making their overexpression in recombinant systems a prerequisite for structural studies. Here, we explore the commonly used eukaryotic expression systems-yeast, baculovirus/insect cells (Sf9) and Semliki Forest Virus (SFV)/mammalian cells-for the expression of seven different eukaryotic membrane proteins from a variety of protein families. The expression levels, quality, biological activity, localization and solubility of all expressed proteins are compared in order to identify the advantages of one system over the other. SFV-transfected mammalian cell lines provide the closest to native environment for the expression of mammalian membrane proteins, and they exhibited the best overall performance. But depending on the protein, baculovirus-infected Sf9 cells performed almost as well as mammalian cells. The lowest expression levels for the proteins tested here were obtained in yeast.
Keywords
Animals, Cell Line, Gene Expression, Genetic Vectors, Humans, Membrane Proteins, Patch-Clamp Techniques, Plasmids, Recombinant Proteins, Solubility
Pubmed
Web of science
Create date
26/02/2009 14:39
Last modification date
20/08/2019 13:57
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