Rhipicephalus microplus serine protease inhibitor family: annotation, expression and functional characterisation assessment

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State: Public
Version: Final published version
Serval ID
serval:BIB_49EA755308B3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Rhipicephalus microplus serine protease inhibitor family: annotation, expression and functional characterisation assessment
Journal
Parasites and Vectors
Author(s)
Rodriguez-Valle M., Xu T., Kurscheid S., Lew-Tabor A.E.
ISSN
1756-3305 (Electronic)
ISSN-L
1756-3305
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
8
Pages
7
Language
english
Notes
Publication types: Research ; research-article Identifiant PubMed Central: PMC4322644
Abstract
BACKGROUND: Rhipicephalus (Boophilus) microplus evades the host's haemostatic system through a complex protein array secreted into tick saliva. Serine protease inhibitors (serpins) conform an important component of saliva which are represented by a large protease inhibitor family in Ixodidae. These secreted and non-secreted inhibitors modulate diverse and essential proteases involved in different physiological processes.
METHODS: The identification of R. microplus serpin sequences was performed through a web-based bioinformatics environment called Yabi. The database search was conducted on BmiGi V1, BmiGi V2.1, five SSH libraries, Australian tick transcriptome libraries and RmiTR V1 using bioinformatics methods. Semi quantitative PCR was carried out using different adult tissues and tick development stages. The cDNA of four identified R. microplus serpins were cloned and expressed in Pichia pastoris in order to determine biological targets of these serpins utilising protease inhibition assays.
RESULTS: A total of four out of twenty-two serpins identified in our analysis are new R. microplus serpins which were named as RmS-19 to RmS-22. The analyses of DNA and predicted amino acid sequences showed high conservation of the R. microplus serpin sequences. The expression data suggested ubiquitous expression of RmS except for RmS-6 and RmS-14 that were expressed only in nymphs and adult female ovaries, respectively. RmS-19, and -20 were expressed in all tissues samples analysed showing their important role in both parasitic and non-parasitic stages of R. microplus development. RmS-21 was not detected in ovaries and RmS-22 was not identified in ovary and nymph samples but were expressed in the rest of the samples analysed. A total of four expressed recombinant serpins showed protease specific inhibition for Chymotrypsin (RmS-1 and RmS-6), Chymotrypsin / Elastase (RmS-3) and Thrombin (RmS-15).
CONCLUSION: This study constitutes an important contribution and improvement to the knowledge about the physiologic role of R. microplus serpins during the host-tick interaction.
Keywords
DNA/genetics, DNA/metabolism, Gene Expression Regulation/physiology, Peptide Hydrolases/metabolism, Rhipicephalus/genetics, Rhipicephalus/metabolism, Saliva/chemistry, Serine Proteinase Inhibitors/genetics, Serine Proteinase Inhibitors/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
25/07/2016 8:18
Last modification date
20/08/2019 13:57
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