CRISPR/Cas9-Mediated Genome Editing for Huntington's Disease

Details

Ressource 1Request a copy Under indefinite embargo.
UNIL restricted access
State: Public
Version: Final published version
License: Not specified
Serval ID
serval:BIB_49CB978C79C5
Type
A part of a book
Publication sub-type
Chapter: chapter ou part
Collection
Publications
Institution
UNIL/CHUV
Title
CRISPR/Cas9-Mediated Genome Editing for Huntington's Disease
Title of the book
Methods in Molecular Biology
Author(s)
Vachey Gabriel, Déglon Nicole
Publisher
Springer New York
ISBN
9781493978243
9781493978250
ISSN
1064-3745
1940-6029
ISSN-L
1064-3745
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
1780
Chapter
21
Pages
463-481
Language
english
Abstract
This chapter describes the potential use of viral-mediated gene transfer in the central nervous system for genome editing in the context of Huntington's disease. Here, we provide protocols that cover the design of various genome editing strategies, the cloning of CRISPR/Cas9 elements into lentiviral vectors, and the assessment of cleavage efficiency, as well as potential unwanted effects.
OAI-PMH
oai:serval.unil.ch:BIB_49CB978C79C5
DOI
10.1007/978-1-4939-7825-0_21
Pubmed
29856031
Web of science
000443467900022
Create date
11/06/2018 9:09
Last modification date
30/07/2024 6:02
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