Characterization of a 67 kDa microtubule-binding protein in the pancreas from different species

Details

Serval ID
serval:BIB_494C041322A8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Characterization of a 67 kDa microtubule-binding protein in the pancreas from different species
Journal
Biochimica et Biophysica Acta (BBA) - General Subjects
Author(s)
Michalik Liliane, Vanier Marie-Thérèse, Launay Jean-François
ISSN
0304-4165
ISSN-L
1872-8006
Publication state
Published
Issued date
06/1992
Peer-reviewed
Oui
Volume
1116
Number
3
Pages
269-273
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Microtubule-interacting proteins have been studied from a pancreas supernatant. These proteins were first identified by affinity chromatography on taxol-stabilized microtubules. Among these interacting polypeptides, we show, for the first time, the presence of a protein which has a molecular mass of 67 kDa, as determined by polyacrylamide slab gel electrophoresis. The heat stability and the ability of this 67 kDa polypeptide to copolymerize with phosphocellulose-purified tubulin suggest that this protein may be a microtubule-associated protein.

Keywords
Animals, Blotting, Western, Cattle, Electrophoresis, Polyacrylamide Gel, Hot Temperature, Microtubule-Associated Proteins/analysis, Microtubule-Associated Proteins/chemistry, Molecular Weight, Pancreas/chemistry, Polymers, Rats, Swine, Tubulin/chemistry
Pubmed
Web of science
Create date
24/01/2008 15:44
Last modification date
20/08/2019 13:56
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