How to confirm C.E.R.A. doping in athletes' blood?

Détails

ID Serval
serval:BIB_47D435872930
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
How to confirm C.E.R.A. doping in athletes' blood?
Périodique
Forensic science international
Auteur(s)
Leuenberger N., Lamon S., Robinson N., Giraud S., Saugy M.
ISSN
1872-6283 (Electronic)
ISSN-L
0379-0738
Statut éditorial
Publié
Date de publication
2011
Volume
213
Numéro
1-3
Pages
101-3
Langue
anglais
Résumé
C.E.R.A. (Continuous Erythropoietin Receptor Activator) is a new third-generation erythropoiesis-stimulating agent that has recently been linked with abuse in endurance sports. The anti-doping community rapidly reacted by releasing a high-throughput screening ELISA allowing the detection of C.E.R.A. doping in athletes' blood. In order to return adverse analytical findings, anti-doping laboratories, however, need, as far as possible, to confirm the presence of the drug in athletes' samples through orthogonal methods. This article focuses on the comparison of 2 proposed confirmation assays based on gel electrophoresis that were coupled with a new sample immunopurification method. IEF, the classical method used to target erythropoietin (EPO) and its recombinant analogues in athletes' samples, and SARKOSYL-PAGE were applied to the plasma samples of subjects having received a single injection of C.E.R.A. It was demonstrated that SARKOSYL-PAGE was at least 6 times more sensitive than IEF, with comparable specificity. A longer detection window coupled with easier interpretation criteria led us to recommend the use of SARKOSYL-PAGE to confirm C.E.R.A. presence in athletes' blood.
Pubmed
Web of science
Création de la notice
15/12/2011 17:19
Dernière modification de la notice
03/03/2018 16:50
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