Clonal imbalances of serum immunoglobulins after allogeneic bone marrow transplantation: an analysis by high-resolution two-dimensional gel electrophoresis

Détails

ID Serval
serval:BIB_47BA98A7DA97
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Clonal imbalances of serum immunoglobulins after allogeneic bone marrow transplantation: an analysis by high-resolution two-dimensional gel electrophoresis
Périodique
Bone Marrow Transplantation
Auteur(s)
Tissot  J. D., Helg  C., Chapuis  B., Zubler  R. H., Jeannet  M., Hochstrasser  D. F., Hohlfeld  P., Schneider  P.
ISSN
0268-3369 (Print)
Statut éditorial
Publié
Date de publication
10/1992
Volume
10
Numéro
4
Pages
347-53
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
The clonality pattern of immunoglobulins (Igs) produced after allogeneic bone marrow transplantation (BMT) was studied by high-resolution two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of serum samples and purified Igs. With this technique, the light (L) chain of a monoclonal Ig usually appears as a single spot. Thus, the degree of clonal diversity of the functional B cells can be appreciated by the electrophoretic pattern of the serum L chains. Furthermore, 2D-PAGE allows a semi-quantitative determination of prominent Ig clones according to the size of L chain spots. We found that serum electrophoretograms of 8/19 patients after BMT (5-9 months) revealed L chain patterns which were similar to those of normal polyclonal Igs, that is, less than five distinguishable small spots among a cloud-like indiscrete L chain spots region ('polyclonal' pattern). A spectrum of clonal abnormalities was observed on the electrophoretograms of 11/19 patients: in five patients, multiple small L chain spots (corresponding to Ig concentrations between 0.2 and 2 g/l) were detected ('oligoclonal' pattern), whereas in six others, 'typical' monoclonal Igs (Ig concentrations > 2 g/l) were observed with (3/19 patients) or without (3/19 patients) multiple small clonal components. Sequential analysis of serum obtained from patients at different times after BMT revealed that imbalanced clonal reconstitution was transient and evolved towards apparently normal polyclonal Ig production. Our observations show that the development of clonal 'gammopathies' after BMT is a frequent, but not obligatory phenomenon. It may reflect a transient restriction of the B cell repertoire either through a limited outgrowth of precursor cells or through selective antigenic pressures.
Mots-clé
Antibody Diversity B-Lymphocytes/immunology Bone Marrow Transplantation/*adverse effects/*immunology Dysgammaglobulinemia/*etiology/immunology Electrophoresis, Gel, Two-Dimensional Humans Immunoglobulin Light Chains/blood Immunoglobulins/*blood Leukemia/immunology/surgery Transplantation, Homologous
Pubmed
Web of science
Création de la notice
25/01/2008 16:34
Dernière modification de la notice
03/03/2018 16:50
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