Isolation and mapping of cDNA hybridization probes specific for ecotropic and nonecotropic murine leukemia proviruses.

Détails

ID Serval
serval:BIB_474D7034DB5A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Isolation and mapping of cDNA hybridization probes specific for ecotropic and nonecotropic murine leukemia proviruses.
Périodique
Virology
Auteur(s)
Herr W., Schwartz D., Gilbert W.
ISSN
0042-6822 (Print)
ISSN-L
0042-6822
Statut éditorial
Publié
Date de publication
1983
Volume
125
Numéro
1
Pages
139-154
Langue
anglais
Résumé
To study the structure of murine leukemia proviruses in AKR mice by Southern hybridization, we have isolated and mapped ecotropic AKV and nonecotropic MCF-derived cDNA restriction fragments. The ecotropic-specific probes originate from four regions of the AKV genome which include the corresponding recombinant region of two MCF viruses (VI-36 and 247). We also isolated two nonecotropic probes from the recombinant region of MCF V1-36. The probes were characterized by (i) mapping of restriction fragments at the 3' end of AKV and MCF V1-36 by a two-dimensional gel strategy, (ii) hybridization of restriction fragments to the related viral RNA genomes followed by electrophoresis, (iii) two-dimensional fingerprinting of single-stranded restriction fragments, and (iv) DNA sequence analysis of the ecotropic probes. The ecotropic AKV and nonecotropic MCF probes discriminate between two populations of endogenous murine leukemia viruses and show that the MCF viruses are not present in the germ line of AKR mice.
Mots-clé
AKR murine leukemia virus/genetics, Base Sequence, DNA/isolation &amp, purification, DNA Restriction Enzymes, Deoxyribonucleases, Type II Site-Specific, Electrophoresis, Polyacrylamide Gel, Genes, Viral, Leukemia Virus, Murine/genetics, Nucleic Acid Hybridization, Recombination, Genetic
Pubmed
Web of science
Création de la notice
24/01/2008 16:36
Dernière modification de la notice
03/03/2018 16:49
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