Imaging of human leukemic T-cell xenografts in nude mice by radiolabeled monoclonal antibodies and F(ab')2 fragments.

Détails

ID Serval
serval:BIB_47159B2567A3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Imaging of human leukemic T-cell xenografts in nude mice by radiolabeled monoclonal antibodies and F(ab')2 fragments.
Périodique
Cancer
Auteur(s)
Vacca A., Buchegger F., Carrel S., Mach J.P.
ISSN
0008-543X (Print)
ISSN-L
0008-543X
Statut éditorial
Publié
Date de publication
1988
Peer-reviewed
Oui
Volume
61
Numéro
1
Pages
58-67
Langue
anglais
Résumé
Monoclonal antibodies (MoAb) that react with the T-lymphocyte markers called cluster of differentiation CD5 and CD2 were labeled with iodine 131 (131I) and were injected intravenously in nude mice bearing solid subcutaneous xenografts derived from the human T-cell leukemia line Ichikawa. Both MoAb anti-CD5 and anti-CD2 yielded favorable mean tumor to whole-body ratios of 3.8 and 5.1, respectively. These ratios were further increased up to 10.0 for MoAb anti-CD5 and 15.5 for MoAb anti-CD2 by using their F(ab')2 fragments. The tumors could be imaged clearly by external scanning after injection of F(ab')2 fragments from both MoAb. F(ab')2 fragments from MoAb anti-CD2 and of a third MoAb recognizing the clonotypic determinant (Ti) of the antigen receptor expressed by the human T-cell line Jurkat were injected in mice bearing intrasplenic Jurkat xenografts. A selective localization of both fragments in tumor tissue was demonstrated with mean tumor to whole-body ratios of 7.5 and 4.1 for MoAb anti-CD2 and anti-Ti, respectively. These in vivo experimental results may provide useful information for the potential use of radiolabeled MoAb and fragments in the diagnosis and treatment of patients with T-cell lymphoma and different other forms of T-cell malignancies.
Mots-clé
Animals, Antibodies, Monoclonal/diagnostic use, Antigens, Differentiation, T-Lymphocyte/analysis, Female, Humans, Immunoglobulin Fab Fragments/immunology, Iodine Radioisotopes/diagnostic use, Leukemia, Lymphoid/immunology, Leukemia, Lymphoid/radionuclide imaging, Mice, Mice, Inbred BALB C, Mice, Nude, Neoplasm Transplantation, T-Lymphocytes/immunology, Transplantation, Heterologous
Pubmed
Web of science
Création de la notice
25/01/2008 12:27
Dernière modification de la notice
03/03/2018 16:49
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