Validation and performance comparison of two carbon isotope ratio methods to control the misuse of androgens in humans.

Détails

ID Serval
serval:BIB_4714C964F147
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Validation and performance comparison of two carbon isotope ratio methods to control the misuse of androgens in humans.
Périodique
Journal of Chromatography. B
Auteur(s)
Saudan C., Emery C., Marclay F., Strahm E., Mangin P., Saugy M.
ISSN
1570-0232
Statut éditorial
Publié
Date de publication
2009
Volume
877
Numéro
23
Pages
2321-2329
Langue
anglais
Résumé
Carbon isotope ratio of androgens in urine specimens is routinely determined to exclude an abuse of testosterone or testosterone prohormones by athletes. Increasing application of gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) in the last years for target and systematic investigations on samples has resulted in the demand for rapid sample throughput as well as high selectivity in the extraction process particularly in the case of conspicuous samples. For that purpose, we present herein the complimentary use of an SPE-based assay and an HPLC fractionation method as a two-stage strategy for the isolation of testosterone metabolites and endogenous reference compounds prior to GC/C/IRMS analyses. Assays validation demonstrated acceptable performance in terms of intermediate precision (range: 0.1-0.4 per thousand) and Bland-Altman analyses revealed no significant bias (0.2 per thousand). For further validation of this two-stage analyses strategy, all the specimens (n=124) collected during a major sport event were processed.
Mots-clé
Androgens/urine, Carbon Isotopes/analysis, Chromatography, Gas/methods, Chromatography, High Pressure Liquid/methods, Doping in Sports, Mass Spectrometry/methods, Substance Abuse Detection/methods, Testosterone/urine
Pubmed
Web of science
Création de la notice
01/10/2009 14:59
Dernière modification de la notice
03/03/2018 16:49
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