Differential stability of antigenic MHC class I-restricted synthetic peptides

Détails

ID Serval
serval:BIB_4712D317BB0B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Differential stability of antigenic MHC class I-restricted synthetic peptides
Périodique
Journal of Immunology
Auteur(s)
Widmann  C., Maryanski  J. L., Romero  P., Corradin  G.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
12/1991
Volume
147
Numéro
11
Pages
3745-51
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 1
Résumé
Various synthetic peptides recognized as Ag by CTL in the context of MHC class I molecules were tested for stability in vitro and in vivo. Peptide inactivation in vitro was quantitated by titrating the amount of peptide required to sensitize target cells for lysis by specific CTL clones. The degree of inactivation after overnight incubation at 37 degrees C varied widely among a series of antigenic peptides. Some were nearly unaffected, whereas others lost activity by more than 100-fold or even 10,000-fold. However, no correlation was found between susceptibility to serum inactivation and antigenic potency as measured in short term cytolytic assays. No inactivation occurred at 4 degrees C, or at 37 degrees C in the absence of serum, under the conditions used. Serum inactivation most likely involved proteolysis because it could be inhibited by protease inhibitors. Moreover, presumed cleavage products of a radiolabeled susceptible peptide could be visualized by TLC. In vivo, the persistence of the antigenic activity of the injected peptides, either in extracellular fluids or on tumor target cells growing in an ascites form, correlated with the degree of stability found for the peptides in vitro. The differential stability of synthetic peptides may have important consequences for attempts to manipulate the development of an immune response in vivo.
Mots-clé
Amino Acid Sequence Animals Antigens, Protozoan/immunology Ascitic Fluid/metabolism Clone Cells Culture Media HLA Antigens/immunology Histocompatibility Antigens Class II/*physiology Mice Molecular Sequence Data Nucleocapsid Proteins *Nucleoproteins Peptides/immunology/*metabolism/pharmacokinetics Protease Inhibitors/pharmacology T-Lymphocytes, Cytotoxic/*immunology Viral Core Proteins/immunology
Pubmed
Web of science
Création de la notice
28/01/2008 12:28
Dernière modification de la notice
03/03/2018 16:49
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