Syntaxin 13 is a developmentally regulated SNARE involved in neurite outgrowth and endosomal trafficking

Détails

ID Serval
serval:BIB_471002E48C96
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Syntaxin 13 is a developmentally regulated SNARE involved in neurite outgrowth and endosomal trafficking
Périodique
European Journal of Neuroscience
Auteur(s)
Hirling  H., Steiner  P., Chaperon  C., Marsault  R., Regazzi  R., Catsicas  S.
ISSN
0953-816X (Print)
Statut éditorial
Publié
Date de publication
06/2000
Volume
12
Numéro
6
Pages
1913-23
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
In addition to its role in exocytosis, SNAP-25 is essential for axonal outgrowth. In order to identify SNARE proteins involved in neurite growth we have used SNAP-25 antibodies to affinity-purify protein complexes enriched in developing rat brain membrane extracts. We have identified a complex between SNAP-25 and syntaxin 13 predominantly present in brain at embryonic or early postnatal stages. We show that syntaxin 13 is developmentally regulated with a decrease in adult brain. In differentiated neuroendocrine PC12 cells as well as primary cortical neurons the protein is localized to a punctated and tubular staining in the perinuclear region and along processes with high levels in the central region of growth cones. Carboxy-terminally tagged syntaxin 13 was also detected on the plasma membrane by in vivo surface-labelling where it colocalized with SNAP-25. Syntaxin 13 has recently been shown to be implicated in early endosomal trafficking. In our study, colocalization with internalized transferrin in the cell body and along neurites confirmed endosomal location in both compartments. Finally, overexpression of full-length syntaxin 13 enhanced neurite outgrowth in NGF-stimulated PC12 cells, whilst it had no effect on regulated secretion. The data suggest that a syntaxin 13-dependent endocytic trafficking step plays a limiting role in membrane expansion during neuronal development.
Mots-clé
Age Factors Amino Acid Sequence Animals Biological Transport/physiology Cell Membrane/chemistry/metabolism Endosomes/chemistry/*metabolism Exocytosis/physiology Growth Cones/chemistry/metabolism Membrane Proteins/analysis/genetics/*metabolism Molecular Sequence Data Nerve Tissue Proteins/analysis/metabolism Neurites/chemistry/*metabolism PC12 Cells Qa-SNARE Proteins Rats SNARE Proteins Synaptosomal-Associated Protein 25 Transfection *Vesicular Transport Proteins
Pubmed
Web of science
Création de la notice
24/01/2008 15:30
Dernière modification de la notice
03/03/2018 16:49
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