Mammalian genes are transcribed with widely different bursting kinetics.

Détails

ID Serval
serval:BIB_46FF0CE7D3AB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Mammalian genes are transcribed with widely different bursting kinetics.
Périodique
Science
Auteur(s)
Suter D.M., Molina N., Gatfield D., Schneider K., Schibler U., Naef F.
ISSN
1095-9203 (Electronic)
ISSN-L
0036-8075
Statut éditorial
Publié
Date de publication
2011
Volume
332
Numéro
6028
Pages
472-474
Langue
anglais
Résumé
In prokaryotes and eukaryotes, most genes appear to be transcribed during short periods called transcriptional bursts, interspersed by silent intervals. We describe how such bursts generate gene-specific temporal patterns of messenger RNA (mRNA) synthesis in mammalian cells. To monitor transcription at high temporal resolution, we established various gene trap cell lines and transgenic cell lines expressing a short-lived luciferase protein from an unstable mRNA, and recorded bioluminescence in real time in single cells. Mathematical modeling identified gene-specific on- and off-switching rates in transcriptional activity and mean numbers of mRNAs produced during the bursts. Transcriptional kinetics were markedly altered by cis-regulatory DNA elements. Our analysis demonstrated that bursting kinetics are highly gene-specific, reflecting refractory periods during which genes stay inactive for a certain time before switching on again.
Mots-clé
Acetylation, Animals, Cells, Cultured, Chromatin/physiology, Circadian Rhythm/genetics, Down-Regulation, Gene Expression, Histones/metabolism, Kinetics, Luminescent Measurements, Mice, Models, Genetic, NIH 3T3 Cells, Promoter Regions, Genetic, Protein Biosynthesis, RNA, Messenger/genetics, RNA, Messenger/metabolism, Stochastic Processes, Transcription, Genetic, Transcriptional Activation, Transgenes, Up-Regulation
Pubmed
Web of science
Création de la notice
28/03/2011 11:13
Dernière modification de la notice
03/03/2018 16:49
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