ROQUIN/RC3H1 alterations are not found in angioimmunoblastic T-cell lymphoma.

Détails

Ressource 1Télécharger: BIB_46F099101268.P001.pdf (429.67 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_46F099101268
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
ROQUIN/RC3H1 alterations are not found in angioimmunoblastic T-cell lymphoma.
Périodique
Plos One
Auteur(s)
Auguste T., Travert M., Tarte K., Amé-Thomas P., Artchounin C., Martin-Garcia N., de Reynies A., de Leval L., Gaulard P., Delfau-Larue M.H.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2013
Volume
8
Numéro
6
Pages
e64536
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: epublish
Résumé
Angioimmunoblastic T-cell Lymphoma (AITL) is one of the most frequent T-cell lymphoma entities. Follicular helper T lymphocytes (TFH) are recognized as the normal cellular counterpart of the neoplastic component. Despite a clonal T-cell feature and few described recurrent cytogenetic abnormalities, a driving oncogenic event has not been identified so far. It has been recently reported that in mice, heterozygous inactivation of Roquin/Rc3h1, a RING type E3 ubiquitine ligase, recapitulates many of the clinical, histological, and cellular features associated with human AITL. In this study we explored whether ROQUIN alterations could be an initial event in the human AITL oncogenic process. Using microarray and RT-PCR analyses, we investigated the levels of ROQUIN transcripts in TFH tumor cells purified from AITL (n = 8) and reactive tonsils (n = 12) and found similar levels of ROQUIN expression in both. Moreover, we also demonstrated that ROQUIN protein was expressed by AITL TFH (PD1+) cells. We then analysed ROQUIN coding sequence in 12 tumor cell-rich AITL samples and found no mutation in any of the samples. Finally, we analysed the expression of MiR101, a putative partner of ROQUIN involved in the modulation of ICOS expression and found similar levels of expression in tumor and reactive TFH. Altogether, this study shows that neither alteration of ROQUIN gene nor deregulation of miR101 expression is likely to be a frequent recurrent event in AITL.
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/08/2013 9:44
Dernière modification de la notice
08/05/2019 17:58
Données d'usage