Rapid detection of MRSA in screening specimens during a hospital outbreak.

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ID Serval
serval:BIB_46DEA102E715
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Abstract (résumé de présentation): article court qui reprend les éléments essentiels présentés à l'occasion d'une conférence scientifique dans un poster ou lors d'une intervention orale.
Collection
Publications
Titre
Rapid detection of MRSA in screening specimens during a hospital outbreak.
Titre de la conférence
20th European Congress of Clinical Microbiology and Infectious (ECCMID)
Auteur(s)
Blanc D., Nahimana-Tessemo I., Jaton-Ogay K.J., Zanetti G.
Adresse
Vienna, Austria, April 10-13, 2010
ISBN
1469-0691
Statut éditorial
Publié
Date de publication
04/2010
Peer-reviewed
Oui
Volume
16
Série
Clinical Microbiology and Infection
Pages
S4
Langue
anglais
Résumé
Objectives: To compare the results of rapid PCR screening for MRSA
using the GeneXpert system with those of cultures in an outbreak setting.
Methods: GeneXpert was used for screening MRSA in nose, throat,
groin, and other clinical samples during a 6-month period. Samples were
performed using a double-swab transystem. When >1 sample was found
positive in a screening set, all second swabs of the set were analysed by
culture.
Results: From June to October 2009, 7568 rapid tests were performed,
among which 432 (5.7%) were positive (nose: 149/2090, 7.1%; throat:
98/2078, 4.7%; groin: 152/2080, 7.3%; urine: 14/1090, 1.3%; wounds:
18/150, 12%; and others:1/27, 3.7%), and 84 (1.1%) were invalid. A
total of 1517 samples were analyzed by both rapid PCR and culture.
Rapid tests had a sensitivity of 0.896 compared to cultures, a specificity
of 0.769, a PPV of 0.763, and a NPV of 0.899. The rapid test was found
to be less sensitive in throat samples (0.81) than in nose or inguinal
samples (0.93 for both). In 32/192 (16%) patients a positive rapid PCR
result was not confirmed by culture, despite several subsequent screening
samples in some patients. Cycle threshold (Ct) for SCCmec of these PCR
positive reactions were all >30.
Conclusions: GeneXpert MRSA was found to be suitable for the rapid
detection in nose, inguinal, and throat samples, however with a lower
sensitivity in the later. Negative cultures in 16% of our PCR-positive
patients raised the question of false positivity or higher sensitivity of
GeneXpert. Further work is needed to investigate these cases.
Création de la notice
10/03/2011 14:52
Dernière modification de la notice
03/03/2018 16:48
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