Sialylation of the sialic acid binding lectin sialoadhesin regulates its ability to mediate cell adhesion.

Détails

ID Serval
serval:BIB_46C91224A518
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Sialylation of the sialic acid binding lectin sialoadhesin regulates its ability to mediate cell adhesion.
Périodique
Blood
Auteur(s)
Barnes Y.C., Skelton T.P., Stamenkovic I., Sgroi D.C.
ISSN
0006-4971[print], 0006-4971[linking]
Statut éditorial
Publié
Date de publication
1999
Volume
93
Numéro
4
Pages
1245-1252
Langue
anglais
Résumé
The macrophage-specific cell surface receptor sialoadhesin, which is a member of the newly recognized family of sialic acid binding lectins called siglecs, binds glycoprotein and glycolipid ligands containing a2-3-linked sialic acid on the surface of several leukocyte subsets. Recently, the sialic acid binding activity of the siglec CD22 has been demonstrated to be regulated by sialylation of the CD22 receptor molecule. In the present work, we show that desialylation of in vivo macrophage sialylconjugates enhances sialoadhesin-mediated lectin activity. Herein, we show that receptor sialylation of soluble sialoadhesin inhibits its binding to Jurkat cell ligands, and that charge-dependent repulsion alone cannot explain this inhibition. Furthermore, we show that the inhibitory effect of sialic acid is partially dependent on the presence of an intact exocyclic side chain. These results, in conjunction with previous findings, suggest that sialylation of siglecs by specific glycosyltransferases may be a common mechanism by which siglec-mediated adhesion is regulated.
Mots-clé
Animals, Antigens, CD/chemistry, Antigens, CD/physiology, Antigens, CD22, Antigens, Differentiation, B-Lymphocyte/chemistry, Antigens, Differentiation, B-Lymphocyte/physiology, COS Cells, Cell Adhesion, Cell Adhesion Molecules, Humans, Jurkat Cells, Lectins, Membrane Glycoproteins/chemistry, Membrane Glycoproteins/physiology, Receptors, Immunologic/chemistry, Receptors, Immunologic/physiology, Sheep, Sialic Acids/chemistry
Pubmed
Création de la notice
26/08/2010 16:42
Dernière modification de la notice
20/08/2019 13:52
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