Global control in Pseudomonas fluorescens mediating antibiotic synthesis and suppression of black root rot of tobacco.

Détails

ID Serval
serval:BIB_46C792DC0249
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Global control in Pseudomonas fluorescens mediating antibiotic synthesis and suppression of black root rot of tobacco.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Laville J., Voisard C., Keel C., Maurhofer M., Défago G., Haas D.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
1992
Volume
89
Numéro
5
Pages
1562-1566
Langue
anglais
Résumé
Pseudomonas fluorescens CHA0 colonizes plant roots, produces several secondary metabolites in stationary growth phase, and suppresses a number of plant diseases, including Thielaviopsis basicola-induced black root rot of tobacco. We discovered that mutations in a P. fluorescens gene named gacA (for global antibiotic and cyanide control) pleiotropically block the production of the secondary metabolites 2,4-diacetylphloroglucinol (Phl), HCN, and pyoluteorin. The gacA mutants of strain CHA0 have a drastically reduced ability to suppress black root rot under gnotobiotic conditions, supporting the previous observations that the antibiotic Phl and HCN individually contribute to the suppression of black root rot. The gacA gene is directly followed by a uvrC gene. Double gacA-uvrC mutations render P. fluorescens sensitive to UV irradiation. The gacA-uvrC cluster is homologous to the orf-2 (= uvrY)-uvrC operon of Escherichia coli. The gacA gene specifies a trans-active 24-kDa protein. Sequence data indicate that the GacA protein is a response regulator in the FixJ/DegU family of two-component regulatory systems. Expression of the gacA gene itself was increased in stationary phase. We propose that GacA, perhaps activated by conditions of restricted growth, functions as a global regulator of secondary metabolism in P. fluorescens.
Mots-clé
Amino Acid Sequence, Anti-Bacterial Agents/biosynthesis, Bacterial Proteins/genetics, Base Sequence, DNA, Bacterial/genetics, Genes, Bacterial, Genes, Regulator, Hydrogen Cyanide/metabolism, Molecular Sequence Data, Mycoses/prevention & control, Operon, Phloroglucinol/analogs & derivatives, Phloroglucinol/metabolism, Plant Diseases/microbiology, Plants, Toxic, Pseudomonas fluorescens/genetics, Restriction Mapping, Tobacco/microbiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 14:51
Dernière modification de la notice
08/05/2019 17:58
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