Detection of Helicobacter pylori and Chlamydia pneumoniae genes in primary orbital lymphoma

Détails

ID Serval
serval:BIB_46BF05499846
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Detection of Helicobacter pylori and Chlamydia pneumoniae genes in primary orbital lymphoma
Périodique
Transactions of the American Ophthalmological Society
Auteur(s)
Chan  C. C., Shen  D., Mochizuki  M., Gonzales  J. A., Yuen  H. K., Guex-Crosier  Y., Lehoang  P.
ISSN
0065-9533
ISSN-L
1545-6110 (Electronic)
Statut éditorial
Publié
Date de publication
2006
Volume
104
Pages
62-70
Notes
Journal Article Research Support, N.I.H., Intramural
Résumé
PURPOSE: Primary orbital non-Hodgkin's lymphoma is a mucosa-associated lymphoid tissue (MALT)-type extranodal marginal zone lymphoma. Chronic antigen stimulation is implicated as a causative agent in the development of some mature B-cell proliferations; for example, there are associations involving Helicobacter pylori with gastric or conjunctival MALT lymphoma and Chlamydia psittaci with ocular adnexal lymphoma. We examined the molecular signatures of H pylori and Chlamydia in eight orbital lymphomas. METHODS: Polymerase chain reaction (PCR) was performed on DNA extracted from microdissected lymphoma cells. H pylori was detected with the urease B and vac/m2 primers. A multiplex touchdown enzyme time-release PCR assay designed to simultaneously detect Chlamydia trachomatis, Chlamydia pneumoniae, and C psittaci was performed. Authenticity of the PCR-amplified products was verified by Southern blot hybridization. RESULTS: H pylori DNA was detected in an orbital lymphoma of a French patient who had positive serum H pylori titer. C pneumoniae, but neither C psittaci nor C trachomatis, DNA was detected in another orbital lymphoma of a Chinese patient from Hong Kong. H pylori, C pneumoniae, and C psittaci genes were not found in the other six orbital lymphomas. CONCLUSION: H pylori or C pneumoniae genomic fingerprints were detected in two of seven primary orbital MALT lymphomas. These findings provide evidence for a possible involvement of particular infectious microorganisms such as H pylori and Chlamydia in primary orbital lymphoma. These different microorganisms may play similar roles in the etiology of orbital MALT lymphomas from different geographic regions. Antibiotic therapy could be considered for orbital MALT lymphomas associated with positive infection.
Mots-clé
Aged, 80 and over Chlamydophila Infections/*microbiology Chlamydophila pneumoniae/*genetics DNA, Bacterial/analysis *Eye Infections, Bacterial/microbiology Female Gene Rearrangement, B-Lymphocyte/genetics Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor/genetics Genes, Bacterial/genetics Genes, T-Cell Receptor gamma/genetics Helicobacter Infections/*microbiology Helicobacter pylori/*genetics Humans Immunoglobulin Heavy Chains/genetics Lymphoma, Mucosa-Associated Lymphoid Tissue/*microbiology/pathology Male Middle Aged Orbital Neoplasms/*microbiology/pathology Polymerase Chain Reaction
Pubmed
Création de la notice
28/01/2008 13:46
Dernière modification de la notice
20/03/2018 13:24
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