Single-Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche-Associated Heterogeneity.

Détails

ID Serval
serval:BIB_46B4FB9DCABE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Single-Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche-Associated Heterogeneity.
Périodique
Immunity
Auteur(s)
Rodda L.B., Lu E., Bennett M.L., Sokol C.L., Wang X., Luther S.A., Barres B.A., Luster A.D., Ye C.J., Cyster J.G.
ISSN
1097-4180 (Electronic)
ISSN-L
1074-7613
Statut éditorial
Publié
Date de publication
15/05/2018
Peer-reviewed
Oui
Volume
48
Numéro
5
Pages
1014-1028.e6
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Résumé
Stromal cells (SCs) establish the compartmentalization of lymphoid tissues critical to the immune response. However, the full diversity of lymph node (LN) SCs remains undefined. Using droplet-based single-cell RNA sequencing, we identified nine peripheral LN non-endothelial SC clusters. Included are the established subsets, Ccl19 <sup>hi</sup> T-zone reticular cells (TRCs), marginal reticular cells, follicular dendritic cells (FDCs), and perivascular cells. We also identified Ccl19 <sup>lo</sup> TRCs, likely including cholesterol-25-hydroxylase <sup>+</sup> cells located at the T-zone perimeter, Cxcl9 <sup>+</sup> TRCs in the T-zone and interfollicular region, CD34 <sup>+</sup> SCs in the capsule and medullary vessel adventitia, indolethylamine N-methyltransferase <sup>+</sup> SCs in the medullary cords, and Nr4a1 <sup>+</sup> SCs in several niches. These data help define how transcriptionally distinct LN SCs support niche-restricted immune functions and provide evidence that many SCs are in an activated state.
Mots-clé
Animals, Chemokine CCL19/genetics, Chemokine CCL19/immunology, Chemokine CCL19/metabolism, Dendritic Cells, Follicular/immunology, Dendritic Cells, Follicular/metabolism, Female, Lymph Nodes/immunology, Lymph Nodes/metabolism, Lymphoid Tissue/cytology, Lymphoid Tissue/immunology, Lymphoid Tissue/metabolism, Mice, Inbred C57BL, Sequence Analysis, RNA/methods, Single-Cell Analysis/methods, Stromal Cells/immunology, Stromal Cells/metabolism, Transcriptome/immunology, double negative cell, fibroblastic reticular cell, follicular dendritic cell, marginal reticular cell, perivascular cell, single-cell RNA sequencing, stromal cell
Pubmed
Web of science
Création de la notice
17/05/2018 19:15
Dernière modification de la notice
09/03/2019 7:26
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