Regulated expression of the trophoblast alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein. Differentiation and cAMP modulate protein and mRNA levels.

Détails

ID Serval
serval:BIB_46B27CF28897
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Regulated expression of the trophoblast alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein. Differentiation and cAMP modulate protein and mRNA levels.
Périodique
Journal of Biological Chemistry
Auteur(s)
Gåfvels M.E., Coukos G., Sayegh R., Coutifaris C., Strickland D.K., Strauss J.F.
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
1992
Volume
267
Numéro
29
Pages
21230-21234
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Résumé
The alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein (alpha 2MR/LRP) has several ligands including activated alpha 2-macroglobulin, pregnancy zone protein, and very low density lipoproteins enriched with apolipoprotein E. The diversity of ligands suggests a role for the alpha 2MR/LRP in a variety of processes including tissue remodeling and lipoprotein metabolism. We examined alpha 2MR/LRP in placental trophoblasts, invasive cells that also function in lipid transport and cholesterol metabolism. alpha 2MR/LRP protein was localized by immunohistochemistry in the syncytiotrophoblast of term placenta. Cytotrophoblasts did not stain prominently. alpha 2MR/LRP (protein and message) in primary cultures of human trophoblast cells increased as cytotrophoblasts differentiated into syncytiotrophoblast. 8-Bromo-cAMP prevented this increase and suppressed alpha 2MR/LRP expression. The cyclic nucleotide had similar suppressive effects on alpha 2MR/LRP in BeWo choriocarcinoma cells. In contrast, low density lipoprotein receptor gene expression was increased. We conclude that: 1) there is a differentiation-dependent pattern of alpha 2MR/LRP expression in the human trophoblast; 2) cAMP negatively regulates alpha 2MR/LRP; 3) there is an inverse relationship between alpha 2MR/LRP and low density lipoprotein receptor gene expression in trophoblast cells.
Mots-clé
Base Sequence, Cell Differentiation, Cells, Cultured, Chorionic Villi/metabolism, Chorionic Villi/ultrastructure, Cyclic AMP/metabolism, Female, Humans, Immunohistochemistry, Low Density Lipoprotein Receptor-Related Protein-1, Molecular Sequence Data, Oligodeoxyribonucleotides, Oligonucleotides, Antisense, Placenta/cytology, Placenta/metabolism, Polymerase Chain Reaction, Pregnancy, RNA, Messenger/metabolism, Receptors, Immunologic/metabolism, Receptors, LDL/genetics, Receptors, LDL/metabolism, Trophoblasts/cytology, Trophoblasts/metabolism
Pubmed
Web of science
Création de la notice
14/10/2014 12:43
Dernière modification de la notice
03/03/2018 16:48
Données d'usage