Interactions of intestinal epithelial cells with bacteria and immune cells: methods to characterize microflora and functional consequences

Détails

ID Serval
serval:BIB_46ABA9E25319
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Interactions of intestinal epithelial cells with bacteria and immune cells: methods to characterize microflora and functional consequences
Périodique
Methods in Molecular Biology
Auteur(s)
Canny  G., Swidsinski  A., McCormick  B. A.
ISSN
1064-3745
Statut éditorial
Publié
Date de publication
2006
Volume
341
Pages
17-35
Notes
Journal Article
Review
Résumé
Epithelial cells at all mucosal surfaces are potentially apposed to bacteria, particularly in the intestine. It is established that intestinal epithelial cells (IECs) represent an important barrier between lamina propria cells and the potentially harmful lumenal contents. In addition, IECs are important immunoeffector cells with the capacity to release cytokines, chemokines, and other molecules involved in antigen presentation and immune defense. The interaction of IECs with intestinal bacteria can result in a decrease in barrier function and the development of inflammation, which is known to be an important factor in the development of intestinal pathology. The potential role of such crosstalk between bacteria and other intestinal cell types in normal physiology and/or pathophysiology is therefore a topic of intense investigation. In this chapter, we provide protocols for the identification of bacteria that are associated with the epithelium and mucosa in addition to functional assays examining the interactions of neutrophils with epithelial cells and epithelial cell-mediated killing of bacteria.
Mots-clé
Animals Antigen Presentation/*immunology Bacteria/*immunology Bacterial Infections/immunology/pathology Bacterial Typing Techniques Cell Communication Cell Line Cytokines/immunology Epithelial Cells/*immunology/pathology Humans *Immunity, Mucosal Inflammation/immunology/pathology Intestinal Mucosa/*immunology/pathology Intestines/*immunology/pathology Mucous Membrane/immunology/pathology
Pubmed
Création de la notice
18/01/2008 13:11
Dernière modification de la notice
03/03/2018 16:48
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