Live Cell Imaging of the Schizosaccharomyces pombe Sexual Life Cycle.

Détails

ID Serval
serval:BIB_464D1553D63F
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Live Cell Imaging of the Schizosaccharomyces pombe Sexual Life Cycle.
Périodique
Cold Spring Harbor protocols
Auteur(s)
Merlini L., Vjestica A., Dudin O., Bendezú F., Martin S.G.
ISSN
1559-6095 (Electronic)
ISSN-L
1559-6095
Statut éditorial
Publié
Date de publication
03/10/2017
Peer-reviewed
Oui
Volume
2017
Numéro
10
Pages
pdb.prot090225
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
The fission yeast <i>Schizosaccharomyces pombe</i> is an invaluable model system for studying the principles that drive sexual differentiation and the meiotic cell division cycle. We describe a simple protocol for microscopic observation of the entire sexual life cycle that can be adapted to focus on specific stages of sexual differentiation. After growth to exponential phase in a nitrogen-rich medium, cell cultures are switched to a nitrogen-deprived medium until the population is enriched for the specific stage of the sexual lifecycle to be studied. Cells are then mounted in easily constructed customized agarose pad chambers for imaging.
Mots-clé
Intravital Microscopy/methods, Meiosis, Microscopy, Fluorescence/methods, Schizosaccharomyces/cytology, Schizosaccharomyces/physiology
Pubmed
Création de la notice
28/08/2017 11:19
Dernière modification de la notice
24/05/2018 19:12
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