Angiotensin II stimulates alpha-skeletal actin expression in cadiomyocytes in vitro and in vivo in the absence of hypertension

Détails

ID Serval
serval:BIB_463EECCFDD13
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Angiotensin II stimulates alpha-skeletal actin expression in cadiomyocytes in vitro and in vivo in the absence of hypertension
Périodique
Differentiation
Auteur(s)
Clement  S., Pellieux  C., Chaponnier  C., Pedrazzini  T., Gabbiani  G.
ISSN
0301-4681 (Print)
Statut éditorial
Publié
Date de publication
12/2001
Volume
69
Numéro
1
Pages
66-74
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
Using a specific alpha-skeletal actin antibody, we have previously shown, that during hypertension-associated cardiac hypertrophy in the rat, the expression of alpha-skeletal actin in the myocardium is increased, but maintains focal distribution, compared to normotensive animals. In the present study, we have investigated whether alpha-skeletal actin expression can be induced in the absence of hypertension. For this purpose, we have examined transgenic mice overexpressing angiotensinogen exclusively in the heart. These animals are characterized by high cardiac angiotensin II levels and cardiac hypertrophy accompanied or not by high blood pressure depending on their genetic background, i.e. presence of one or two renin genes. Alpha-skeletal actin levels were highly increased in transgenic compared to wild-type myocardium independently of the number of renin genes, indicating that angiotensin II can stimulate alpha-skeletal actin expression in normotensive animals. Additional in vitro experiments using cultured mouse and rat cardiomyocytes showed that angiotension II not only increases alpha-skeletal actin expression but also induces an increase of its incorporation within II-bands compared to control cardiomyocytes. Angiotensin II increases also the expression of alpha-smooth muscle actin in sarcomeres of cardiomyocytes as well as in fibroblastic cells present within the culture.
Mots-clé
Actins/*metabolism Angiotensin II/*physiology Animals Base Sequence Cell Differentiation/*physiology DNA Primers Hypertension/*metabolism Mice Mice, Transgenic Myocardium/cytology/*metabolism Rats Rats, Wistar Renin/genetics
Pubmed
Web of science
Création de la notice
25/01/2008 9:45
Dernière modification de la notice
03/03/2018 16:47
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