Biodiversity of amoebae and amoeba-resisting bacteria in a hospital water network

Détails

ID Serval
serval:BIB_463324FB4DF9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Biodiversity of amoebae and amoeba-resisting bacteria in a hospital water network
Périodique
Applied and Environmental Microbiology
Auteur(s)
Thomas  V., Herrera-Rimann  K., Blanc  D. S., Greub  G.
ISSN
0099-2240
Statut éditorial
Publié
Date de publication
04/2006
Peer-reviewed
Oui
Volume
72
Numéro
4
Pages
2428-38
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Résumé
Free-living amoebae (FLA) are ubiquitous organisms that have been isolated from various domestic water systems, such as cooling towers and hospital water networks. In addition to their own pathogenicity, FLA can also act as Trojan horses and be naturally infected with amoeba-resisting bacteria (ARB) that may be involved in human infections, such as pneumonia. We investigated the biodiversity of bacteria and their amoebal hosts in a hospital water network. Using amoebal enrichment on nonnutrient agar, we isolated 15 protist strains from 200 (7.5%) samples. One thermotolerant Hartmannella vermiformis isolate harbored both Legionella pneumophila and Bradyrhizobium japonicum. By using amoebal coculture with axenic Acanthamoeba castellanii as the cellular background, we recovered at least one ARB from 45.5% of the samples. Four new ARB isolates were recovered by culture, and one of these isolates was widely present in the water network. Alphaproteobacteria (such as Rhodoplanes, Methylobacterium, Bradyrhizobium, Afipia, and Bosea) were recovered from 30.5% of the samples, mycobacteria (Mycobacterium gordonae, Mycobacterium kansasii, and Mycobacterium xenopi) were recovered from 20.5% of the samples, and Gammaproteobacteria (Legionella) were recovered from 5.5% of the samples. No Chlamydia or Chlamydia-like organisms were recovered by amoebal coculture or detected by PCR. The observed strong association between the presence of amoebae and the presence of Legionella (P < 0.001) and mycobacteria (P = 0.009) further suggests that FLA are a reservoir for these ARB and underlines the importance of considering amoebae when water control measures are designed.
Mots-clé
Alphaproteobacteria/classification/genetics/growth & development/isolation & purification Amoeba/classification/genetics/*isolation & purification/microbiology Animals Bacteria/classification/genetics/growth & development/*isolation & purification Coculture Techniques Culture Media Fresh Water/*microbiology/*parasitology Genotype *Hospitals Molecular Sequence Data Phenotype Phylogeny Sequence Analysis, DNA *Water Supply
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/01/2008 16:20
Dernière modification de la notice
08/05/2019 17:56
Données d'usage