Biologically addressable monolayer structures formed by templates of sulfur-bearing molecules

Details

Serval ID
serval:BIB_4631FC389BDC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Biologically addressable monolayer structures formed by templates of sulfur-bearing molecules
Journal
Biophysical Journal
Author(s)
Duschl  C., Liley  M., Corradin  G., Vogel  H.
ISSN
0006-3495 (Print)
Publication state
Published
Issued date
09/1994
Volume
67
Number
3
Pages
1229-37
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Abstract
We demonstrate that the combined application of Langmuir-Blodgett and self-assembly techniques allows the fabrication of patterns with contrasting surface properties on gold substrates. The process is monitored using fluorescence microscopy and surface plasmon spectroscopy and microscopy. These structures are suitable for the investigation of biochemical processes at surfaces and in ultrathin films. Two examples of such processes are shown. In the first example, the structures are addressed through the binding of a monoclonal antibody to a peptide. This demonstrates the formation of self-assembled monolayers by cysteine-bearing peptides on gold, and the directed binding of proteins to the structured layers. A high contrast between specific and unspecific binding of proteins is observed by the patterned presentation of antigens. Such films possess considerable potential for the design of multichannel sensor devices. In the second example, a structured phospholipid layer is produced by controlled self-assembly from vesicle solution. The structures created--areas of phospholipid bilayer, surrounded by a matrix of phospholipid monolayer--allow formation of a supported bilayer which is robust and strongly bound to the gold support, with small areas of free-standing bilayer which very closely resemble a phospholipid cell membrane.
Keywords
Amino Acid Sequence Biophysics Lipid Bilayers/chemistry Membrane Proteins/chemistry *Membranes, Artificial Microscopy, Fluorescence Models, Chemical Molecular Sequence Data Palmitic Acid Palmitic Acids/chemistry Peptides/chemistry Sulfur/chemistry Surface Properties
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:55
Last modification date
20/08/2019 13:51
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