Biologically addressable monolayer structures formed by templates of sulfur-bearing molecules

Détails

ID Serval
serval:BIB_4631FC389BDC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Biologically addressable monolayer structures formed by templates of sulfur-bearing molecules
Périodique
Biophysical Journal
Auteur(s)
Duschl  C., Liley  M., Corradin  G., Vogel  H.
ISSN
0006-3495 (Print)
Statut éditorial
Publié
Date de publication
09/1994
Volume
67
Numéro
3
Pages
1229-37
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
We demonstrate that the combined application of Langmuir-Blodgett and self-assembly techniques allows the fabrication of patterns with contrasting surface properties on gold substrates. The process is monitored using fluorescence microscopy and surface plasmon spectroscopy and microscopy. These structures are suitable for the investigation of biochemical processes at surfaces and in ultrathin films. Two examples of such processes are shown. In the first example, the structures are addressed through the binding of a monoclonal antibody to a peptide. This demonstrates the formation of self-assembled monolayers by cysteine-bearing peptides on gold, and the directed binding of proteins to the structured layers. A high contrast between specific and unspecific binding of proteins is observed by the patterned presentation of antigens. Such films possess considerable potential for the design of multichannel sensor devices. In the second example, a structured phospholipid layer is produced by controlled self-assembly from vesicle solution. The structures created--areas of phospholipid bilayer, surrounded by a matrix of phospholipid monolayer--allow formation of a supported bilayer which is robust and strongly bound to the gold support, with small areas of free-standing bilayer which very closely resemble a phospholipid cell membrane.
Mots-clé
Amino Acid Sequence Biophysics Lipid Bilayers/chemistry Membrane Proteins/chemistry *Membranes, Artificial Microscopy, Fluorescence Models, Chemical Molecular Sequence Data Palmitic Acid Palmitic Acids/chemistry Peptides/chemistry Sulfur/chemistry Surface Properties
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:55
Dernière modification de la notice
08/05/2019 17:56
Données d'usage