A dynamic view of hepatitis C virus replication complexes.

Détails

ID Serval
serval:BIB_461F52384BFA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A dynamic view of hepatitis C virus replication complexes.
Périodique
Journal of Virology
Auteur(s)
Wölk B., Büchele B., Moradpour D., Rice C.M.
ISSN
1098-5514
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
82
Numéro
21
Pages
10519-10531
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
Hepatitis C virus (HCV) replicates its genome in a membrane-associated replication complex (RC). Specific membrane alterations, designated membranous webs, represent predominant sites of HCV RNA replication. The principles governing HCV RC and membranous web formation are poorly understood. Here, we used replicons harboring a green fluorescent protein (GFP) insertion in nonstructural protein 5A (NS5A) to study HCV RCs in live cells. Two distinct patterns of NS5A-GFP were observed. (i) Large structures, representing membranous webs, showed restricted motility, were stable over many hours, were partitioned among daughter cells during cell division, and displayed a static internal architecture without detectable exchange of NS5A-GFP. (ii) In contrast, small structures, presumably representing small RCs, showed fast, saltatory movements over long distances. Both populations were associated with endoplasmic reticulum (ER) tubules, but only small RCs showed ER-independent, microtubule (MT)-dependent transport. We suggest that this MT-dependent transport sustains two distinct RC populations, which are both required during the HCV life cycle.
Mots-clé
Cell Line, Endoplasmic Reticulum, Genes, Reporter, Green Fluorescent Proteins, Hepacivirus, Humans, Microscopy, Fluorescence, Recombinant Fusion Proteins, Staining and Labeling, Viral Nonstructural Proteins, Virus Replication
Pubmed
Web of science
Open Access
Oui
Création de la notice
02/03/2009 14:52
Dernière modification de la notice
08/05/2019 17:56
Données d'usage