Characterization of mixed chimerism in patients with chronic myeloid leukemia transplanted with T-cell-depleted bone marrow: involvement of different hematologic lineages before and after relapse

Détails

ID Serval
serval:BIB_45D6A1789B1C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Characterization of mixed chimerism in patients with chronic myeloid leukemia transplanted with T-cell-depleted bone marrow: involvement of different hematologic lineages before and after relapse
Périodique
Blood
Auteur(s)
Roux  E., Abdi  K., Speiser  D., Helg  C., Chapuis  B., Jeannet  M., Roosnek  E.
ISSN
0006-4971 (Print)
Statut éditorial
Publié
Date de publication
01/1993
Volume
81
Numéro
1
Pages
243-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 1
Résumé
We have characterized mixed chimerism (MC) in five patients with chronic myeloid leukemia (CML) who received transplants with T-cell-depleted bone marrow (BM) and who relapsed within 4 years after transplantation. To study the possible relation of MC with relapse, we purified different populations of leukocytes and analyzed their donor/recipient origin by a method based on polymerase chain reaction amplification of minisatellite DNA regions. Our results show that before relapse, all hematopoietic recipient cells are T cells, whereas monocytes, B, and natural killer (NK) cells are of donor origin. This observation does not appear to be specific for CML as similar results were found in two control patients with acute myeloid leukemia (AML). At the time of (CML) relapse, recipient granulocytes, monocytes, and erythrocytes appeared and progressively replaced the respective lineages of donor origin. No other lineages seemed to be involved as B cells and NK cells remained of donor origin and no significant changes in the number of recipient T cells were detected. In this respect relapse of CML after BM transplantation (BMT) seems not to be very different from the primary disease in chronic phase before transplantation. Furthermore, we conclude that after BMT, an association between mixed chimerism before relapse and the (CML) relapse does exist because both phenomena are consequences of T-cell depletion of the BM graft. However, this correlation might well be indirect as the MC caused by the recipient T cells appears to be independent of the one caused by the recurrent disease.
Mots-clé
Adult B-Lymphocytes/immunology/pathology Bone Marrow/pathology *Bone Marrow Purging *Bone Marrow Transplantation Cell Separation *Chimera Erythrocytes/pathology Female Granulocytes/pathology Humans Immunophenotyping Killer Cells, Natural/immunology/pathology Leukemia, Myeloid, Chronic/pathology/*surgery Leukemia, Myeloid, Philadelphia-Positive/pathology Male Middle Aged Neoplasm Recurrence, Local/*pathology T-Lymphocytes/immunology/*pathology Tissue Donors
Pubmed
Web of science
Création de la notice
28/01/2008 12:33
Dernière modification de la notice
03/03/2018 16:46
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