Characterization of mixed chimerism in patients with chronic myeloid leukemia transplanted with T-cell-depleted bone marrow: involvement of different hematologic lineages before and after relapse
Details
Serval ID
serval:BIB_45D6A1789B1C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Characterization of mixed chimerism in patients with chronic myeloid leukemia transplanted with T-cell-depleted bone marrow: involvement of different hematologic lineages before and after relapse
Journal
Blood
ISSN
0006-4971 (Print)
Publication state
Published
Issued date
01/1993
Volume
81
Number
1
Pages
243-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 1
Research Support, Non-U.S. Gov't --- Old month value: Jan 1
Abstract
We have characterized mixed chimerism (MC) in five patients with chronic myeloid leukemia (CML) who received transplants with T-cell-depleted bone marrow (BM) and who relapsed within 4 years after transplantation. To study the possible relation of MC with relapse, we purified different populations of leukocytes and analyzed their donor/recipient origin by a method based on polymerase chain reaction amplification of minisatellite DNA regions. Our results show that before relapse, all hematopoietic recipient cells are T cells, whereas monocytes, B, and natural killer (NK) cells are of donor origin. This observation does not appear to be specific for CML as similar results were found in two control patients with acute myeloid leukemia (AML). At the time of (CML) relapse, recipient granulocytes, monocytes, and erythrocytes appeared and progressively replaced the respective lineages of donor origin. No other lineages seemed to be involved as B cells and NK cells remained of donor origin and no significant changes in the number of recipient T cells were detected. In this respect relapse of CML after BM transplantation (BMT) seems not to be very different from the primary disease in chronic phase before transplantation. Furthermore, we conclude that after BMT, an association between mixed chimerism before relapse and the (CML) relapse does exist because both phenomena are consequences of T-cell depletion of the BM graft. However, this correlation might well be indirect as the MC caused by the recipient T cells appears to be independent of the one caused by the recurrent disease.
Keywords
Adult
B-Lymphocytes/immunology/pathology
Bone Marrow/pathology
*Bone Marrow Purging
*Bone Marrow Transplantation
Cell Separation
*Chimera
Erythrocytes/pathology
Female
Granulocytes/pathology
Humans
Immunophenotyping
Killer Cells, Natural/immunology/pathology
Leukemia, Myeloid, Chronic/pathology/*surgery
Leukemia, Myeloid, Philadelphia-Positive/pathology
Male
Middle Aged
Neoplasm Recurrence, Local/*pathology
T-Lymphocytes/immunology/*pathology
Tissue Donors
Pubmed
Web of science
Create date
28/01/2008 12:33
Last modification date
20/08/2019 14:50