Characterization of a monoclonal antibody to cis-urocanic acid: detection of cis-urocanic acid in the serum of irradiated mice by immunoassay

Détails

ID Serval
serval:BIB_45ABF4AE5F1B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Characterization of a monoclonal antibody to cis-urocanic acid: detection of cis-urocanic acid in the serum of irradiated mice by immunoassay
Périodique
Immunology
Auteur(s)
Moodycliffe  A. M., Norval  M., Kimber  I., Simpson  T. J.
ISSN
0019-2805 (Print)
Statut éditorial
Publié
Date de publication
08/1993
Volume
79
Numéro
4
Pages
667-72
Notes
Journal Article --- Old month value: Aug
Résumé
Cis-urocanic acid (cis-UCA), which is formed from the naturally occurring trans-isomer on ultraviolet (UV) irradiation, has been suggested as a photoreceptor for and mediator of the suppressive effects of UV irradiation on systemic immune responses. Trans-UCA is located predominantly in the stratum corneum, and the extent of isomerization to cis-UCA may be analysed by high-performance liquid chromatography (HPLC) of skin extracts. Such an analysis is not suitable for other tissues. In this study a murine monoclonal antibody to cis-UCA was prepared and tested by ELISA using UCA isomers conjugated to protein as antigens. The interaction of the antibody with structural analogues of UCA was assessed by competitive inhibition ELISA which indicated that the antibody had a high specificity for cis-UCA. Screening of sera at various times after UVB irradiation of mice by competitive inhibition ELISA using the monoclonal antibody showed that cis-UCA was present, probably in an unbound form, for at least 2 days after the exposure. Thus, cis-UCA produced in the epidermis following UVB irradiation reaches the serum a few hours later. The implications of this finding for the generation of suppressed immune responses are discussed.
Mots-clé
Animals Antibodies, Monoclonal/*immunology Antibody Specificity/immunology Antigens/immunology Female Immune Tolerance Isomerism Mice Mice, Inbred BALB C Serum Albumin, Bovine/immunology *Ultraviolet Rays Urocanic Acid/analogs & derivatives/*blood/immunology
Pubmed
Web of science
Création de la notice
25/01/2008 17:50
Dernière modification de la notice
03/03/2018 16:46
Données d'usage