Differentiation of postmitotic neuroblasts into substance P-immunoreactive sensory neurons in dissociated cultures of chick dorsal root ganglion.

Détails

ID Serval
serval:BIB_45A4996F49C1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Differentiation of postmitotic neuroblasts into substance P-immunoreactive sensory neurons in dissociated cultures of chick dorsal root ganglion.
Périodique
Developmental biology
Auteur(s)
Barakat I., Droz B.
ISSN
0012-1606
Statut éditorial
Publié
Date de publication
1987
Peer-reviewed
Oui
Volume
122
Numéro
1
Pages
274-286
Langue
anglais
Résumé
Counts performed on dissociated cell cultures of E10 chick embryo dorsal root ganglia (DRG) showed after 4-6 days of culture a pronounced decline of the neuronal population in neuron-enriched cultures and a net gain in the number of ganglion cells in mixed DRG cell cultures (containing both neurons and nonneuronal cells). In the latter case, the increase in the number of neurons was found to depend on NGF and to average 119% in defined medium or 129% in horse serum-supplemented medium after 6 days of culture. The lack of [3H]thymidine incorporation into the neuronal population indicated that the newly formed ganglion cells were not generated by proliferation. On the contrary, the differentiation of postmitotic neuroblasts present in the nonneuronal cell compartment was supported by sequential microphotographs of selected fields taken every hour for 48-55 hr after 3 days of culture. Apparently nonneuronal flat dark cells exhibited morphological changes and gradually evolved into neuronal ovoid and refringent cell bodies with expanding neurites. The ultrastructural organization of these evolving cells corresponded to that of primitive or intermediate neuroblasts. The neuronal nature of these rounding up cell bodies was indeed confirmed by the progressive expression of various neuronal cell markers (150 and 200-kDa neurofilament triplets, neuron specific enolase, and D2/N-CAM). Besides a constant lack of immunoreactivity for tyrosine hydroxylase, somatostatin, parvalbumin, and calbindin-D 28K and a lack of cytoenzymatic activity for carbonic anhydrase, all the newly produced neurons expressed three main phenotypic characteristics: a small cell body, a strong immunoreactivity to MAG, and substance P. Hence, ganglion cells newly differentiated in culture would meet characteristics ascribed to small B sensory neurons and more specifically to a subpopulation of ganglion cells containing substance P-immunoreactive material.
Mots-clé
Acetylcholinesterase, Animals, Cell Count, Cell Differentiation, Cells, Cultured, Chick Embryo, Ganglia, Spinal, Microscopy, Electron, Mitosis, Myelin Proteins, Myelin-Associated Glycoprotein, Neurons, Afferent, Substance P
Pubmed
Web of science
Création de la notice
30/03/2009 10:27
Dernière modification de la notice
03/03/2018 16:46
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