Expression on human thymocytes of the idiotypic structures (Ti) from two leukemia T cell lines Jurkat and HPB-ALL.

Détails

ID Serval
serval:BIB_4582213DD356
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Expression on human thymocytes of the idiotypic structures (Ti) from two leukemia T cell lines Jurkat and HPB-ALL.
Périodique
European journal of immunology
Auteur(s)
Carrel S., Isler P., Schreyer M., Vacca A., Salvi S., Giuffre L., Mach J.P.
ISSN
0014-2980
Statut éditorial
Publié
Date de publication
1986
Peer-reviewed
Oui
Volume
16
Numéro
6
Pages
649-652
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The expression on a significant number of thymocytes of idiotypic structures (Ti) restricted to HPB-ALL or Jurkat cells is demonstrated. As many as 2-4% of thymocytes were stained with anti-Ti HPB-ALL or anti-Ti Jurkat monoclonal antibodies, when analyzed by flow microfluorometry. Immunohistochemical localization studies performed on frozen thymus specimens of either fetal or pediatric origin indicated a scattered distribution of Ti-positive cells in both the cortex and the medulla. From lysates of 125I-labeled pediatric thymocytes, anti-Ti HPB-ALL and anti-Ti Jurkat monoclonal antibodies precipitated disulfide-linked heterodimers comparable to those precipitated from 125I-labeled HPB-ALL or Jurkat cells as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis.
Mots-clé
Antibodies, Monoclonal/immunology, Antibodies, Neoplasm/immunology, Antigen-Antibody Reactions, Antigens, Neoplasm/analysis, Cell Line, Humans, Immunoglobulin Idiotypes/analysis, Immunoglobulin Idiotypes/immunology, Leukemia, Lymphoid/analysis, Leukemia, Lymphoid/immunology, Precipitin Tests, Staining and Labeling, T-Lymphocytes/analysis, T-Lymphocytes/immunology
Pubmed
Web of science
Création de la notice
19/11/2009 13:48
Dernière modification de la notice
03/03/2018 16:45
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